Microorganisms for the production of aniline

Abstract

A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline.

What is claimed is: 
     
         1 . A non-naturally occurring microbial organism, comprising a microbial organism having an aniline pathway comprising at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline, said aniline pathway comprising an anthranilate synthase and an anthranilate decarboxylase. 
     
     
         2 . The non-naturally occurring microbial organism of  claim 1 , further comprising a DAHP synthase. 
     
     
         3 . The non-naturally occurring microbial organism of  claim 1 , further comprising an enzyme selected from a 3-dehydroquinate synthase, a 3-dehydroquinate dehydratase, a shikimate dehydrogenase or a quinate/shikimate dehydrogenase, a shikimate kinase, a 3-phosphoshikimate-1-carboxyvinyltransferase, and a chorismate synthase. 
     
     
         4 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises two exogenous nucleic acids each encoding an aniline pathway enzyme. 
     
     
         5 . The non-naturally occurring microbial organism of  claim 4 , wherein said two exogenous nucleic acids encode an anthranilate synthase and an anthranilate decarboxylase. 
     
     
         6 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises three exogenous nucleic acids each encoding an aniline pathway enzyme. 
     
     
         7 . The non-naturally occurring microbial organism of  claim 6 , wherein said three exogenous nucleic acids encode a DAHP synthase, an anthranilate synthase and an anthranilate decarboxylase. 
     
     
         8 . The non-naturally occurring microbial organism of  claim 1 , wherein said microbial organism comprises four exogenous nucleic acids each encoding an aniline pathway enzyme. 
     
     
         9 . The non-naturally occurring microbial organism of  claim 8 , wherein said four exogenous nucleic acids encode a 3-dehydroquinate synthase, a DAHP synthase, an anthranilate synthase and an anthranilate decarboxylase. 
     
     
         10 . The non-naturally occurring microbial organism of  claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid. 
     
     
         11 . The non-naturally occurring microbial organism of  claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.