PCP2 Mini-Promoters

Abstract

Isolated polynucleotides comprising a PCP2 mini-promoter are provided. The mini-promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, gene therapy, etc.

What is claimed is: 
     
         1 . An isolated polynucleotide comprising a PCP2 regulatory element with at least 95% sequence identity to SEQ ID NO: 3 in a reverse orientation from the natural genomic configuration, operably linked to a PCP2 basal promoter with at least 95% sequence identity to SEQ ID NO: 2, wherein the spacing between the PCP2 regulatory element and the PCP2 basal promoter is not more than 500 nucleotides. 
     
     
         2 . The isolated polynucleotide of  claim 1 , operably linked to an expressible sequence. 
     
     
         3 . A vector comprising the isolated polynucleotide of  claim 1 . 
     
     
         4 . A cell comprising the vector of  claim 3 . 
     
     
         5 . The cell of  claim 4 , wherein the vector is stably integrated into the genome of the cell. 
     
     
         6 . The cell of  claim 4 , wherein the cell is a stem cell, neuronal cell, or a retinal cell. 
     
     
         7 . A method of expressing a sequence of interest, the method comprising operably linking the sequence of interest to the polynucleotide of  claim 1 ; and introducing the operably linked sequence of interest into a cell permissive for expression from the PCP2 mini-promoter. 
     
     
         8 . The isolated polynucleotide of  claim 1 , comprising the sequence of SEQ ID NO:1 and wherein the spacing between the PCP2 regulatory element and the PCP2 basal promoter is not more than 100 nucleotides.