Polymer particle containing fluorescent molecule and method for producing the same

Abstract

A method for preparing polymer magnetic particles comprising magnetic particles and a coating polymer layer covering the magnetic particles and containing fluorescent molecules, the method includes swelling a coating polymer layer of the polymer magnetic particles in a non-aqueous solvent-containing and absorbing the fluorescent molecules inside the swelled coating polymer layer, adding water to the non-aqueous solvent in which the polymer magnetic particles adsorbing the fluorescent molecules are present, and removing the non-aqueous solvent by evaporation; where the non-aqueous solvent has affinity with water, a lower boiling point, and a higher vapor pressure than those of water, and the fluorescent molecule is selected from rare earth metal chelate complexes.

1 . A method for preparing a polymer magnetic particle containing fluorescent molecules in an amount of at least 100 nmol of fluorescent molecules per 1 mg of the polymer magnetic particles, the polymer magnetic particle comprising magnetic particles and a coating polymer layer covering the magnetic particles and containing the fluorescent molecules, the method comprising:
 swelling a coating polymer layer of the polymer magnetic particles in a non-aqueous solvent-containing therein the fluorescent molecules and absorbing the fluorescent molecules inside the swelled coating polymer layer;   adding water to the non-aqueous solvent in which the polymer magnetic particles adsorbing the fluorescent molecules are present; and   removing the non-aqueous solvent by evaporation;   wherein the non-aqueous solvent has affinity with water, a lower boiling point, and a higher vapor pressure than those of water, and the fluorescent molecule is selected from rare earth metal chelate complexes.   
     
     
         2 . A method for preparing a polymer magnetic particle containing at least 100 nmol of fluorescent molecules per 1 mg of the polymer magnetic particles, the polymer magnetic particle comprising magnetic particles and a coating polymer layer covering the magnetic particles and containing the fluorescent molecules, the method comprising:
 removing water by solvent substitution from an aqueous dispersion of polymer magnetic particles with a non-aqueous solvent and then swelling a coating polymer layer of polymer magnetic particles in a non-aqueous solvent dissolving therein fluorescent molecules and absorbing the fluorescent molecules inside the swelled coating polymer layer;   adding water to the non-aqueous solvent in which the polymer magnetic particles absorbing the fluorescent molecules are present; and   removing the non-aqueous solvent by evaporation;   wherein the non-aqueous solvent has affinity with water, a lower boiling point, and a higher vapor pressure than those of water, and the fluorescent molecule is selected from rare earth metal chelate complexes.   
     
     
         3 . The method of  claim 1 , wherein the coating polymer layer is formed by a polymer consisting of a monomer ingredient selected from styrene and glycidyl methacrylate. 
     
     
         4 . The method of  claim 1 , wherein the rare earth metal consisting of the rare earth metal chelate complexes are selected from the group consisting of Europium, Samarium, Terbium and Dysprosium.