Multi-Enzymatic Preparation Containing the Secretome of an Aspergillus Japonicus Strain

Abstract

The invention relates to a multi-enzymatic preparation containing the secretome of the CNCM I-4639 strain of Aspergillus japonicus . This secretome, which contains in particular cellulases and hemicellulases, can be used for the saccharification of lignocellulosic substrates, in particular in combination with the secretome of Trichoderma reesei.

1 . A multi-enzyme preparation containing cellulases and hemicellulases, characterized in that it contains the secretome of the strain CNCM I-4639 from  Aspergillus japonicus.    
     
     
         2 . The preparation as claimed in  claim 1 , characterized in that said secretome is obtained from a culture of the strain CNCM I-4639 prepared in the presence of a source of carbon that induces the production of lignocellulolytic enzymes, wherein the source of carbon contains arabinoxylans. 
     
     
         3 . The preparation as claimed in  claim 2 , characterized in that said inductive source of carbon is chosen from cereal brans, fractions of cereal brans, or mixtures thereof. 
     
     
         4 . The multi-enzyme preparation as claimed in  claim 1 , characterized in that it also contains the secretome of a strain of  Trichoderma reesei.    
     
     
         5 . A method for saccharification of a lignocellulosic substrate, characterized in that the substrate is contacted with a multi-enzyme preparation as claimed in  claim 1 . 
     
     
         6 . A process for producing fermentable sugars from a lignocellulosic substrate, characterized in that the process comprises hydrolysis of said substrate using a multi-enzyme preparation as claimed in  claim 1 . 
     
     
         7 . A process for producing alcohol from a lignocellulosic substrate, characterized in that the process comprises production of a hydrolyzate containing fermentable sugars via a process as claimed in  claim 6 , and the alcoholic fermentation of said hydrolyzate by an alcohol-producing microorganism. 
     
     
         8 . The multi-enzyme preparation as claimed in  claim 2 , characterized in that it also contains the secretome of a strain of  Trichoderma reesei.    
     
     
         9 . The multi-enzyme preparation as claimed in  claim 3 , characterized in that it also contains the secretome of a strain of  Trichoderma reesei.    
     
     
         10 . A method for saccharification of a lignocellulosic substrate, characterized in that the substrate is contacted with a multi-enzyme preparation as claimed in  claim 2 . 
     
     
         11 . A method for saccharification of a lignocellulosic substrate, characterized in that the substrate is contacted with a multi-enzyme preparation as claimed in  claim 3 . 
     
     
         12 . A method for saccharification of a lignocellulosic substrate, characterized in that the substrate is contacted with a multi-enzyme preparation as claimed in  claim 4 . 
     
     
         13 . A process for producing fermentable sugars from a lignocellulosic substrate, characterized in that the process comprises hydrolysis of said substrate using a multi-enzyme preparation as claimed in  claim 2 . 
     
     
         14 . A process for producing fermentable sugars from a lignocellulosic substrate, characterized in that the process comprises hydrolysis of said substrate using a multi-enzyme preparation as claimed in  claim 3 . 
     
     
         15 . A process for producing fermentable sugars from a lignocellulosic substrate, characterized in that the process comprises hydrolysis of said substrate using a multi-enzyme preparation as claimed in  claim 4 . 
     
     
         16 . The process for producing alcohol of  claim 7  from a lignocellulosic substrate, characterized in that hydrolysis of said substrate uses a preparation containing a secretome obtained from a culture of the strain CNCM I-4639 prepared in the presence of a source of carbon that induces the production of lignocellulolytic enzymes, wherein the source of carbon containing contains arabinoxylans. 
     
     
         17 . The process as claimed in  claim 16 , characterized in that said inductive source of carbon is chosen from cereal brans, and fractions of cereal brans, or mixtures thereof. 
     
     
         18 . The process as claimed in  claim 16 , characterized in that the preparation also contains the secretome of a strain of  Trichoderma reesei.