Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Abstract

The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

1 . Process for preparing a sugar product from ligno-cellulosic material, comprising:
 a) optionally pre-treatment of the ligno-cellulosic material;   b) optionally washing of the optionally pre-treated ligno-cellulosic material;   c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and   d) optionally recovery of a sugar product;   wherein after the pre-treatment and before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.   
     
     
         2 . Process for preparing a fermentation product from ligno-cellulosic material, comprising:
 a) optionally pre-treatment of the ligno-cellulosic material;   b) optionally washing of the optionally pre-treated ligno-cellulosic material;   c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61;   d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and   e) optionally recovery of a fermentation product;   wherein after the pre-treatment and before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.   
     
     
         3 . Process according to  claim 1 , wherein during the enzymatic hydrolysis c) oxygen is added to the ligno-cellulosic material. 
     
     
         4 . Process according to  claim 1 , wherein the oxygen is added in the form of bubbles. 
     
     
         5 . Process according to  claim 1 , wherein the reactor for the enzymatic hydrolysis has a volume of 1 m 3  or more. 
     
     
         6 . Process according to  claim 2 , wherein the enzymatic hydrolysis time is 5 to 150 hours. 
     
     
         7 . Process according to  claim 1 , wherein the enzyme composition used retains activity for 30 hours or more. 
     
     
         8 . Process according to  claim 1 , wherein the hydrolysis is conducted at a temperature of 45° C. or more, optionally 50° C. or more and optionally at a temperature of 55° C. or more. 
     
     
         9 . Process according to  claim 1 , wherein the
 enzyme composition is derived from a fungus, optionally microorganism of the genus  Rasamsonia  or the enzyme composition comprises a fungal enzyme, a  Rasamsonia  enzyme.   
     
     
         10 . Process according to  claim 1 , wherein the dry matter content in the hydrolysis c) is 10 wt % or more, optionally is 14 wt % or more and optionally is 14 to 33 wt %. 
     
     
         11 . Process according to  claim 9 , wherein the dry matter content in the hydrolysis c) is 14 to 33% wt %. 
     
     
         12 . A process according to  claim 1  in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor. 
     
     
         13 . A process according to  claim 1  in which oxygen is introduced as an oxygen-containing gas optionally air.