Method of making collagen powder from marine cartilage and skin

Abstract

A method of making hydrolyzed marine Type II collagen includes the mixing of marine cartilage, water, an enzyme and a protease enzyme for an extended period of time. Once mixed, the mixture is heated for a period of time at 150° F. Once heated, the enzymes are deactivated, the bone sediment separated, and the fat removed. Next, maltodextrin is added to the mixture and finally the mixture is spray dried to form a collagen powder.

1 . A method of making hydrolyzed marine collagen Type II, comprising the steps of:
 mixing marine cartilage, water, an enzyme and a protease enzyme to form a mixture in a digestion tank;   heating the mixture at a temperature of approximately 150° F.;   deactivating the enzyme and the protease enzyme by adjusting the temperature to between 180° F. and 200° F.;   separating bone sediment from the mixture;   removing fat from the mixture and heating the mixture to between 140° F. and 170° F.;   adding maltodextrin to the mixture; and   spray drying the mixture to form a collagen powder.   
     
     
         2 . The method of  claim 1  wherein the marine cartilage is shark cartilage. 
     
     
         3 . The method of  claim 2  wherein between 500 and 3,000 pounds of shark cartilage is mixed. 
     
     
         4 . The method of  claim 2  wherein 1,000 pounds of shark cartilage is mixed. 
     
     
         5 . The method of  claim 1  wherein between 500 and 2,500 pounds of water is provide for mixing. 
     
     
         6 . The method of  claim 1  wherein 1,025 pounds of water is provided for mixing. 
     
     
         7 . The method of  claim 1  wherein the enzyme is a papain enzyme in a range of between 500 mLs to 20 L. 
     
     
         8 . The method of  claim 1  wherein the enzyme added for mixing is 4,800 mLs of 200 T.U. 
     
     
         9 . The method of  claim 1  wherein the protease enzyme added for mixing is between 10 mLs and 20 L. 
     
     
         10 . The method of  claim 1  wherein the protease enzyme added for mixing is 14 L. 
     
     
         11 . The method of  claim 1  wherein the step of heating the mixture at a temperature of 150° F. is performed for a time period between 30 minutes and 48 hours. 
     
     
         12 . The method of  claim 1 , further comprising the steps of drying and grinding the bone sediment. 
     
     
         13 . The method of  claim 1  wherein the step of adding maltodextrin to the mixture includes a quantity of between 1% and 60% per weight of the mixture is added to the tank. 
     
     
         14 . The method of  claim 13  wherein the quantity added is between 30% and 32% weight of the mixture. 
     
     
         15 . The method of  claim 1  wherein the collagen powder has a molecular weight between 18,000 and 180,000 Daltons. 
     
     
         16 . The method of  claim 1  wherein the collagen powder includes a minimum of 4% hyaluronic acid. 
     
     
         17 . The method of  claim 1  wherein the collagen powder includes a minimum of 5% chondroitin. 
     
     
         18 . The method of  claim 1  wherein the collagen powder includes between 2% and 45% of mucopolysaccharide. 
     
     
         19 . The method of  claim 1  wherein the collagen powder has a minimum of 10% protein. 
     
     
         20 . A method of making hydrolyzed marine Type I collagen, comprising the steps of:
 combining water, marine skin, and sodium hydroxide to form a mixture in a tank;   mixing the tank for approximately 40 minutes;   rinsing the mixture;   adding water and sulfuric acid to the tank and mixing for approximately 40 minutes;   draining liquid from the tank while retaining the marine skin; and   adding citric acid and water to the tank and mixing for approximately 40 minutes.