Topical halobacteria extract composition for treating radiation skin tissue damage

Abstract

A composition for treating skin damage that includes halobacteria extracts and Daunaliella extracts. The aforementioned extracts have strong antioxidants with high redox potential when dissolved in oil and in water. The strong antioxidants inhibit known oxidative mechanisms which are further correlated with skin damage. The halobacteria extract is Archaebacteria DN-1, which has a wide range impact on rehabilitation of the skin tissue after radiation. Furthermore, the composition is preferably adapted for topical delivery.

What is claimed is: 
     
         1 . A composition for treating skin damage comprising halobacteria extracts, said halobacteria extracts comprising strong antioxidants with high redox potential when dissolved in oil and in water; said strong antioxidants inhibiting known oxidative mechanisms correlated with skin damage;
 wherein said halobacteria extract is Archaebacteria DN-1 which has a wide range impact on rehabilitation of the skin tissue after radiation; said composition is adapted for topical delivery.   
     
     
         2 . The composition according to  claim 1 , wherein said composition further comprises  Daunaliella  extracts. 
     
     
         3 . A composition for treating skin damage in a mammalian subject, said composition comprising: a Archaebacteria DN-1 halobacterial extracts, said extracts comprising:
 a. at least one water soluble fraction; and   b. at least one oil soluble fraction;   
       wherein said Archaebacteria DN-1 provides an anti oxidant activity as measured by any of in vitro Assay 1-4; said activity substantially correlated with a body serum TAC of at least 167.1 μMol Trolox Equivalent/100 g measured by one selected from a group consisting of:
 a. oxygen radical absorbent capacity (ORAC) Assay 1 at the treatment site; 
 b. ferric reducing ability of plasma (FRAP) Assay 4 at the treatment site; 
 c. 2,2-diphenyl-1-picrylhydrazyl (DPPH) Assay 4 at the treatment site; 
 d. 3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) Assay 4 at the treatment site; 
 e. Trolox Equivalent Antioxidant Capacity (TEAC) Assay 5 at the treatment site; and 
 f. any combination thereof. 
 
     
     
         4 . The composition according to  claim 3 , wherein said body serum measurement is taken from any body site. 
     
     
         5 . The composition according to  claim 3 , wherein said assay 1-5 measurement of total antioxidant activity is performed in a serum sample of said composition or in an object plasma tissue. 
     
     
         6 . The composition according to  claim 1 , wherein said halobacteria extracts:
 a. provides antioxidant and redox potential to a wound site, correllated with an increase in erythrocytes (RBC), leukocytes (WBC), Interleukin-6 (IL-6) concentration and glutathione concentration in a treated subject compared with an untreated control;   b. protects membranes from oxidation by reacting with radicals produced in the chain reaction.   
     
     
         7 . The composition according to  claim 1 , further wherein said composition is for decreasing of clinical syndromes resulting from radiation in variety of systems such as the nerve system, digesting system and vascular system. 
     
     
         8 . The composition according to  claim 1 , further wherein said composition is for treating cardiac, liver or vascular diseases, stabilization and improvement of the immune system and the endocrine system. 
     
     
         9 . The composition according to  claim 1 , wherein said composition comprises by weight, 2.5%-10% Archaebacteria DN-1. 
     
     
         10 . The composition according to  claim 1 , wherein said topical delivery is selected from the group consisting of: a gel, a milk, a lotion, a serum, a mask, ointments or a cream. 
     
     
         11 . The composition according to  claim 1 , wherein said skin damage is selected from the group consisting of: skin blemish, scars, burns, mucositis or/and bedsores resulting from radiation treatment, surgery or any drug treatment. 
     
     
         12 . A skin damage treatment composition comprising halobacterial extract, promotes rehabilitation of the body tissue after radiation, alleviation of depression, reduction of clinical syndromes resulting from radiation, treatment of cardiac, liver or vascular diseases, stabilization and improvement of the immune system and the endocrine system. 
     
     
         13 . A skin damage treatment composition comprising halobacterial extract, further comprises, preservatives, surfactants, humectants, emulsifiers, thickening agents, perfumes, preservatives, vegetable or mineral oils, antiseptic agents, acidifying or alkalinizing agents, vitamins, anti-UV agents, solvents, pH-stabilizing agents, silicones and combination thereof. 
     
     
         14 . A method of producing an Archaebacteria DN-1 composition comprising the steps of:
 a. obtaining a bacterial mass by culturing halobacteria;   b. dispersing a quantity of the bacterial mass in a solvent to form a solution;   c. extracting the solution with a halogenated solvent;   d. extracting the solution with a C1-C4 alkanol; and   e. extracting the solution with water;   
       wherein said halobacteria extract is Archaebacteria DN-1 having an anti oxidant activity which has a wide range impact on rehabilitation of the skin tissue after radiation; said activity substantially correlated with a body serum TAC of at least 167.1 μMol Trolox Equivalent/100 g at the treatment site. 
     
     
         15 . The method according to  claim 14 , further wherein said composition is for one selected from a group consisting of:
 a. decreasing of clinical syndromes resulting from radiation in variety of systems such as the nerve system, digesting system and vascular system;   b. treating cardiac, liver or vascular diseases, stabilization and improvement of the immune system and the endocrine system; and   c. any combination thereof.   
     
     
         16 . The method according to  claim 14 , wherein said composition comprises by weight, 2.5%-10% Archaebacteria DN-1, 
     
     
         17 . The method according to  claim 14 , wherein the composition treats skin blemish such as scars, burns or/and bedsores resulting from radiation, surgery or any drug treatment. 
     
     
         18 . The method according to  claim 14 , wherein said composition comprising halobacterial extract, promotes rehabilitation of the body tissue after radiation, alleviation of depression, reduction of clinical syndromes resulting from radiation, treatment of cardiac, liver or vascular diseases, stabilization and improvement of the immune system and the endocrine system. 
     
     
         19 . The method according to  claim 14 , wherein said composition further comprises: preservatives, surfactants, humectants, emulsifiers, thickening agents, perfumes, preservatives, vegetable or mineral oils, antiseptic agents, acidifying or alkalinizing agents, vitamins, anti-UV agents, solvents, pH-stabilizing agents, silicones and combination thereof. 
     
     
         20 . The method according to  claim 14 , wherein said halobacteria extract has antioxidant activity due to its redox properties, resulting in increment of erythrocytes (RBC), leukocytes (WBC), Interleukin-6 (IL-6) concentration and glutathione concentration.