US2015197769A1PendingUtilityA1
Methods for Enhancing Stress Tolerance in Plants and Compositions Thereof

Assignee: MONSANTO TECHNOLOGY LLCPriority: Sep 29, 2003Filed: Mar 30, 2015Published: Jul 16, 2015
Est. expirySep 29, 2023(expired)· nominal 20-yr term from priority
Inventors:Mary Fernandes
C12N 15/8261C12N 15/8273A01H 1/02C12N 15/82A01H 1/06
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References
Claims
Abstract

Increased tolerance to abiotic stress in a plant is provided by introducing DNA expressing a cold shock protein, e.g. bacterial cold shock protein.
Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 .- 20 . (canceled) 
     
     
         21 . A drought tolerant transgenic plant that comprises in its genome a recombinant DNA molecule that expresses a cold shock protein that comprises the cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3 and that confers resistance to drought. 
     
     
         22 . The drought tolerant transgenic plant of  claim 21 , wherein said cold shock protein is a bacterial or a plant cold shock protein. 
     
     
         23 . The drought tolerant transgenic plant of  claim 21 , wherein said cold shock protein has at least 60% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         24 . The drought tolerant transgenic plant of  claim 21 , wherein said cold shock protein has at least 90% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         25 . The drought tolerant transgenic plant of  claim 21 , wherein said cold shock protein is selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         26 . The drought tolerant transgenic plant of  claim 21 , wherein said transgenic plant is a monocot or a dicot plant. 
     
     
         27 . The drought tolerant transgenic plant of  claim 21 , wherein said transgenic plant is a soybean, corn, canola, rice, cotton, barley, oat, turf grass, alfalfa, or wheat plant. 
     
     
         28 . The transgenic plant of  claim 21 , wherein said plant has an increased yield when compared to a non-transformed plant of the same species when said transgenic plant and said non-transformed plant are grown under drought stress. 
     
     
         29 . A transgenic propagule of the drought tolerant transgenic plant of  claim 21 , wherein said propagule comprises in its genome a recombinant DNA molecule that expresses a cold shock protein that comprises the cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3 and that confers drought resistance to a transgenic plant comprising said recombinant DNA that is produced from said propagule. 
     
     
         30 . The transgenic propagule of  claim 29 , wherein said cold shock protein has at least 60% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         31 . The transgenic propagule of  claim 29 , wherein said cold shock protein has at least 90% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         32 . The transgenic propagule of  claim 29 , wherein said propagule is a seed. 
     
     
         33 . The transgenic propagule of  claim 29 , wherein said propagule is a root, shoot, leaf, stem, embryo, or cell. 
     
     
         34 . A method of improving drought tolerance in a transgenic progeny plant comprising: (i) crossing a plant with a drought tolerant transgenic plant having a recombinant DNA expressing a cold shock protein that comprises a cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3 and that confers resistance to drought; and (ii) obtaining a transgenic progeny plant having said recombinant DNA. 
     
     
         35 . The method of  claim 34 , wherein said transgenic plant is a soybean, corn, canola, rice, cotton, barley, oat, turf grass, alfalfa, or wheat plant. 
     
     
         36 . A method of producing a drought tolerant transgenic plant comprising the steps of:
 a) inserting into the genome of plant cells a recombinant DNA molecule that comprises, in the 5′ to 3′ direction:
 (i) a first DNA polynucleotide comprising a promoter that functions in plants and which is operably linked to 
 (ii) a second DNA polynucleotide that encodes a cold shock protein that comprises a cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3, which is operably linked to 
 (iii) a 3′ transcription termination DNA polynucleotide that functions as a polyadenylation sequence; 
   b) obtaining transformed plant cells containing said recombinant DNA;   c) regenerating transgenic plants from said plant cells; and   d) selecting a transgenic plant having increased drought tolerance.   
     
     
         37 . The method of  claim 36 , wherein said cold shock protein has at least 60% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         38 . The method of  claim 36 , wherein said cold shock protein has at least 80% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         39 . The method of  claim 36 , wherein said cold shock protein has at least 90% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         40 . The method of  claim 36 , wherein said cold shock protein is selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         41 . The method of  claim 36 , wherein said transgenic plant is a monocot or a dicot plant. 
     
     
         42 . The method of  claim 36 , wherein said transgenic plant is a soybean, corn, canola, rice, cotton, barley, oat, turf grass, alfalfa, or wheat plant. 
     
     
         43 . The method of  claim 36 , wherein said transgenic plant has an increased yield when compared to a non-transformed plant of the same species and when said transgenic plant and said non-transformed plant are grown under drought stress. 
     
     
         44 . A method for increasing yield in a crop subject to water deficit during its growth, said method comprising planting seeds having a recombinant DNA expressing a cold shock protein that comprises the cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3 and that confers resistance to drought, and allowing said seeds to grow to mature plants under drought conditions. 
     
     
         45 . The method of  claim 44 , wherein said cold shock protein has at least 60% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         46 . The method of  claim 44 , wherein said cold shock protein has at least 80% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         47 . The method of  claim 44 , wherein said cold shock protein has at least 90% identity across the entire length of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         48 . The method of  claim 44 , wherein said cold shock protein is selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 47, SEQ ID NO: 49, and SEQ ID NO: 51. 
     
     
         49 . The method of  claim 44 , wherein said plants are soybean, corn, canola, rice, cotton, barley, oat, turf grass, alfalfa, or wheat plants.
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