Hydroxytrosol containing extract obtained from olives and solids containing residues of olive oil extraction

Abstract

Hydroxytyrosol extracted from olives and/or from the solid residues of olives after the extraction of olive oil, by acid hydrolysis and purification on resin columns eluted with water contains hydroxytyrosol and tyrosol, is free from sugars, has a residual content of Benzo[a]pyrene that is less than 2 microg/Kg (weight BaP/weight of extract as dry matter), containing a weight ratio of hydroxytyrosol to hydroxymethylfurfural of between 45:1 and 10000:1, and the content of hydroxytyrosol in the extract is at least 0.5% (w/w) with purity of at least 40% (by HPLC 280 nm).

1 .- 15 . (canceled) 
     
     
         16 . A dietary supplement comprising an olive extract containing a weight ratio of hydroxytyrosol to oleuropein greater than 200:1. 
     
     
         17 . A dietary supplement comprising an olive extract containing a weight ratio of hydroxytyrosol to aldehydic form of oleuropein aglycone of between 5:1 and 850:1. 
     
     
         18 . A dietary supplement comprising an olive extract containing a weight ratio of hydroxytyrosol to tyrosol of between 55:1 and 618:1. 
     
     
         19 . An oral composition comprising olive polyphenols in an amount effective to provide in a daily serving at least 5 mg of hydroxytyrosol and its derivatives wherein said olive polyphenols comprise a weight ratio of hydroxytyrosol to tyrosol of between 55:1 and 618:1. 
     
     
         20 . A method of protecting blood lipids in a subject from oxidative stress, wherein the method comprises administering to the subject the oral composition according to  claim 19 . 
     
     
         21 . An oral composition comprising olive polyphenols in an amount effective to provide in a daily serving at least 5 mg of hydroxytyrosol and its derivatives wherein said olive polyphenols comprise a weight ratio of hydroxytyrosol to aldehydic form of oleuropein aglycone of between 5:1 and 850:1. 
     
     
         22 . A method of protecting blood lipids in a subject from oxidative stress, wherein the method comprises administering to the subject the oral composition according to  claim 21 . 
     
     
         23 . An oral composition comprising olive polyphenols in an amount effective to provide in a daily serving at least 5 mg of hydroxytyrosol and its derivatives wherein said olive polyphenols comprise a weight ratio of hydroxytyrosol to oleuropein greater than 200:1. 
     
     
         24 . A method of protecting blood lipids in a subject from oxidative stress, wherein the method comprises administering to the subject the oral composition according to  claim 23 . 
     
     
         25 . The dietary supplement of  claim 16 , additionally comprising a carrier. 
     
     
         26 . The dietary supplement of  claim 17 , additionally comprising a carrier. 
     
     
         27 . The dietary supplement of  claim 18 , additionally comprising a carrier. 
     
     
         28 . The oral composition of  claim 19 , additionally comprising a carrier. 
     
     
         29 . The oral composition of  claim 21 , additionally comprising a carrier. 
     
     
         30 . The oral composition of  claim 23 , additionally comprising a carrier.