Paramyxovirus and Methods of Use

Abstract

The present invention is directed towards a novel virus, called Cedar Virus, and its methods of use.

1 - 11 . (canceled) 
     
     
         12 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of
 a) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:2,   b) a polypeptide comprising the amino acid sequence of SEQ ID NO:2,   c) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2,   d) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:3,   e) a polypeptide comprising the amino acid sequence of SEQ ID NO:3,   f) a polypeptide consisting of the amino acid sequence of SEQ ID NO:3,   g) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:4,   h) a polypeptide comprising the amino acid sequence of SEQ ID NO:4,   i) a polypeptide consisting of the amino acid sequence of SEQ ID NO:4,   j) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:5,   k) a polypeptide comprising the amino acid sequence of SEQ ID NO:5,   l) a polypeptide consisting of the amino acid sequence of SEQ ID NO:5,   m) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:6,   n) a polypeptide comprising the amino acid sequence of SEQ ID NO:6,   o) a polypeptide consisting of the amino acid sequence of SEQ ID NO:6,   p) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:7,   q) a polypeptide comprising the amino acid sequence of SEQ ID NO:7,   r) a polypeptide consisting of the amino acid sequence of SEQ ID NO:7,   s) a polypeptide with an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:8   t) a polypeptide comprising the amino acid sequence of SEQ ID NO:8, and   u) a polypeptide consisting of the amino acid sequence of SEQ ID NO:8.   
     
     
         13 . An isolated polynucleotide encoding the polypeptide of  claim 12 , or the complement thereof. 
     
     
         14 . The polynucleotide of  claim 13 , wherein the polynucleotide is RNA. 
     
     
         15 . The polynucleotide of  claim 14 , wherein the molecule is DNA. 
     
     
         16 . A vector comprising the nucleic acid molecule of  claim 15 . 
     
     
         17 . A host cell comprising the vector of  claim 16 . 
     
     
         18 . The host cell of  claim 17 , wherein the host cell is a prokaryotic cell. 
     
     
         19 . The host cell of  claim 17 , wherein the host cell is a eukaryotic cell. 
     
     
         20 . The host cell of  claim 19 , wherein the host cell is a mammalian cell. 
     
     
         21 . A method for producing a polypeptide, the method comprising culturing the host cell of  claim 17  in conditions that promote protein production and isolating the polypeptide from the culture. 
     
     
         22 . An antibody or fragment thereof that specifically binds to the polypeptide of  claim 12 . 
     
     
         23 . The antibody of  claim 22 , wherein the antibody is a monoclonal antibody or fragment thereof, a polyclonal antibody or a fragment thereof, a humanized antibody or a fragment thereof, a recombinantly produced antibody or a fragment thereof or a chimeric antibody or a fragment thereof. 
     
     
         24 . A vaccine comprising the antibody or fragment of  claim 22 . 
     
     
         25 . A method of treating or preventing an infection of Hendra virus, Nipah virus, Cedar virus, or other paramyxoviruses in a subject, the method comprising administering the vaccine of  claim 24  to a subject in need or treatment or prevention thereof. 
     
     
         26 . A method of detecting the presence of a first polynucleotide in a sample, wherein the first polynucleotide comprises a nucleotide sequence of at least 100 contiguous nucleotides of SEQ ID NO:1, the method comprising contacting the sample with a binding molecule that selectively binds to at least a portion of the first polynucleotide, or the complement thereof, and detecting the binding of the binding molecule to the first polynucleotide. 
     
     
         27 . The method of  claim 26  wherein the first polynucleotide comprises a nucleotide sequence that is at least 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 11000, 12000, 13000, 14000, 15000, 16000, 17000 or 18000 contiguous nucleotides of SEQ ID NO:1. 
     
     
         28 . The method of  claim 26  wherein the binding molecule is a second polynucleotide comprising at least 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 nucleotides in length. 
     
     
         29 . The method of  claim 26  wherein the first polynucleotide is amplified using PCR or RT-PCR prior to detection. 
     
     
         30 . A recombinant virus comprising the polynucleotide of  claim 12 . 
     
     
         31 . The recombinant virus of  claim 30  wherein the virus is an attenuated virus.