Novel cardiomyocyte marker

Abstract

The present invention provides a method for producing or detecting cardiomyocytes, which method comprises extracting/detecting cardiomyocytes from a cell population comprising cardiomyocytes using, as an index, positivity of NCAM1, SSEA3, SSEA4 and/or CD340.

1 . A method for producing a cardiomyocyte(s), comprising
 a) providing a cell population comprising the cardiomyocyte(s), and   b) extracting a cardiomyocyte(s) from the cell population comprising the cardiomyocyte(s) using, as an index, positivity of at least one selected from the group consisting of NCAM1, SSEA3, SSEA4 and CD340.   
     
     
         2 . The method according to  claim 1 , wherein said cardiomyocyte is human cardiomyocyte. 
     
     
         3 . The method according to  claim 1 , wherein said cell population comprising cardiomyocyte(s) is a cell population obtained by inducing differentiation of a pluripotent stem cell(s), or a cell population composed of cells of an isolated tissue(s). 
     
     
         4 . The method according to  claim 3 , wherein said pluripotent stem cell is an ES cell or iPS cell. 
     
     
         5 . The method according to  claim 3 , wherein said induction of differentiation into a cardiomyocyte(s) comprises forming an embryoid body. 
     
     
         6 . The method according to  claim 4 , wherein said induction of differentiation into a cardiomyocyte(s) comprises culturing an embryoid body in a medium comprising a cytokine(s). 
     
     
         7 . The method according to  claim 6 , wherein said cytokine(s) is at least one cytokine selected from the group consisting of activin A, BMP4, b-FGF, and VEGF. 
     
     
         8 . The method according to  claim 6 , wherein said medium further comprises a Wnt inhibitor. 
     
     
         9 . The method according to  claim 8 , wherein said Wnt inhibitor is DKK-1. 
     
     
         10 . A method for detecting a cardiomyocyte(s), comprising detecting a cardiomyocyte(s) in a cell population comprising a cardiomyocyte using, as an index, positivity of at least one selected from the group consisting of NCAM1, SSEA3, SSEA4 and CD340. 
     
     
         11 . The method according to  claim 10 , wherein said cardiomyocyte is human cardiomyocyte. 
     
     
         12 . The method according to  claim 10 , wherein said cell population comprising a cardiomyocyte is a cell population obtained by inducing differentiation of a pluripotent stem cell(s), or a cell population composed of cells of an isolated tissue(s). 
     
     
         13 . The method according to  claim 12 , wherein said pluripotent stem cell is an ES cell or iPS cell. 
     
     
         14 . A kit for extracting or detecting a cardiomyocyte(s), comprising a reagent for detecting at least one selected from the group consisting of NCAM1, SSEA3, SSEA4 and CD340. 
     
     
         15 . The kit according to  claim 14 , wherein said cardiomyocyte is a human cardiomyocyte.