Affinity support and method for trapping substance using the same

Abstract

Problems to be Solved The present invention provides an affinity support capable of trapping a substance by cooperative binding that is less likely to Surface of cause dissociation even when the substance is a molecule other than an antibody, and a trapping method using the same. Means to Solve the Problems A method of trapping a substance comprising the step of contacting an objective to be trapped with an affinity support comprising a support, a spacer bound to the support and an affinity substance bound to the spacer, so as to bind the objective to be trapped to the affinity substance, wherein each one of the objective to be trapped has a plural of affinity sites and the affinity substance binds to at least two of the affinity sites simultaneously.

1 . A method of trapping a substance comprising the step of contacting an objective to be trapped with an affinity support comprising a support, a spacer bound to the support and an affinity substance bound to the spacer, so as to bind the objective to be trapped to the affinity substance, wherein each one of the objective to be trapped has a plural of affinity sites and the affinity substance binds to at least two of the affinity sites simultaneously. 
     
     
         2 . The method of trapping a substance according to  claim 1 , wherein one kind of the affinity substance bound to the spacer exists on the affinity support. 
     
     
         3 . The method of trapping a substance according to  claim 1 , wherein the spacer comprises an oxyalkylene group. 
     
     
         4 . The method of trapping the substance according to  claim 1 , wherein the spacer is selected from the group consisting of a polyalkylene glycol, a polyoxyalkylated polyol, a polyvinyl alcohol, a polyvinyl alkyl ether, a polysaccharide, a biodegradable polymer, and a lipopolymer. 
     
     
         5 . The method of trapping a substance according to  claim 1 , wherein each one of the objective to be trapped is one multimer or one aggregate which comprises a plural of molecules, and each of the plural of the affinity sites exists on each of the plural of the molecules. 
     
     
         6 . The method of trapping a substance according to  claim 1 , wherein each one of the objective to be trapped is one molecule. 
     
     
         7 . The method of trapping a substance according to  claim 1 , wherein the objective to be trapped is a biological substance or a non-biological substance. 
     
     
         8 . The method of trapping a substance according to  claim 7 , wherein the biological substance is selected from the group consisting of a biomarker, a biomarker candidate molecule, and a fragment generated from a biomarker or a biomarker candidate molecule by splicing. 
     
     
         9 . The method of trapping a substance according to  claim 8 , wherein the biomarker is related to a disease selected from the group consisting of cancer, brain disease, heart disease, immune disease, hepatic disease, renal disease, and eye disease.