US2015174577A1PendingUtilityA1

Filler Fluids for Droplet Operations

56
Assignee: ADVANCED LIQUID LOGIC INCPriority: Apr 18, 2006Filed: Mar 5, 2015Published: Jun 25, 2015
Est. expiryApr 18, 2026(expired)· nominal 20-yr term from priority
Inventors:Vijay SrinivasanMichael G. PollackPhilip PaikVamsee K. PamulaRichard B. Fair
B01L 2400/0427B81B 1/00B01L 3/50273B03C 5/02B01L 3/502792B01L 3/502784B01L 7/52B01L 7/54B01L 2300/0816B01L 2300/089B01F 33/3021G01N 2035/1046B01L 2200/10B01L 2300/0861B01L 3/502761B01L 2300/1827B01L 7/525B01L 3/502715B01L 2200/0673B01L 2200/147B01L 2200/0647B01L 2400/0448G01N 27/447B01F 33/3031
56
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Claims

Abstract

The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.

Claims

exact text as granted — not AI-modified
1 - 45 . (canceled) 
     
     
         46 . A droplet microactuator comprising:
 (a) a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate;   (b) a second substrate spaced from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and the second substrate; and   (c) a droplet and a filler fluid in the interior volume, wherein the droplet is surrounded by the filler fluid, and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes, wherein the filler fluid comprises an oil and a surfactant, and wherein the surfactant comprises nonionic low hydrophile-lipophile balanced (HLB) surfactant with an HLB less than about 10.   
     
     
         47 . The droplet microactuator of  claim 46  wherein the filler fluid comprises one or more silicone oils. 
     
     
         48 . The droplet microactuator of  claim 46  wherein the filler fluid comprises one or more fluorosilicone oils. 
     
     
         49 . The droplet microactuator of  claim 46  wherein the filler fluid comprises one or more hydrocarbons. 
     
     
         50 . The droplet microactuator of  claim 46  wherein the filler fluid comprises one or more alkanes. 
     
     
         51 . The droplet microactuator of  claim 46  wherein the filler fluid comprises a component selected from the group consisting of decane, undecane, dodecane, tridecane, tetradecane, pentadecane, hexadecane. 
     
     
         52 . The droplet microactuator of  claim 46  wherein the filler fluid comprises a component selected from the group consisting of aromatic alkanes. 
     
     
         53 . The droplet microactuator of  claim 46  wherein the filler fluid comprises a mineral oil. 
     
     
         54 . The droplet microactuator of  claim 46  wherein the filler fluid comprises a paraffin oil. 
     
     
         55 . The droplet microactuator of  claim 46  wherein the filler fluid is solid at room temperature of about 25° C. 
     
     
         56 . The droplet microactuator of  claim 46  further comprising a second filler fluid surrounding or in contact with the filler fluid. 
     
     
         57 . The droplet microactuator of  claim 46  wherein the HLB is less than about 5. 
     
     
         58 . The droplet microactuator of  claim 46  wherein the droplet comprises whole blood and the surfactant has an HLB which is less than about 2. 
     
     
         59 . The droplet microactuator of  claim 46  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         60 . The droplet microactuator of  claim 46  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         61 . The droplet microactuator of  claim 46  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         62 . The droplet microactuator of  claim 46  wherein the filler fluid comprises silicone oil and the surfactant is a nonionic lipophile surfactant in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         63 . The droplet microactuator of  claim 46  wherein the filler fluid comprises silicone oil and the surfactant is a nonionic lipophile surfactant in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         64 . The droplet microactuator of  claim 46  wherein the filler fluid comprises silicone oil and the surfactant is a nonionic lipophile surfactant in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         65 . A droplet microactuator comprising:
 (a) a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate;   (b) a second substrate spaced from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and the second substrate; and   (c) a droplet and a filler fluid in the interior volume, wherein the droplet is surrounded by the filler fluid, and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes, wherein the filler fluid comprises an oil and a surfactant, and wherein the surfactant is a nonionic low hydrophile-lipophile balanced (HLB) surfactant or a nonionic lipophile surfactant.   
     
     
         66 . The droplet microactuator of  claim 65  wherein the filler fluid comprises one or more silicone oils. 
     
     
         67 . The droplet microactuator of  claim 65  wherein the filler fluid comprises one or more fluorosilicone oils. 
     
     
         68 . The droplet microactuator of  claim 65  wherein the filler fluid comprises one or more hydrocarbons. 
     
     
         69 . The droplet microactuator of  claim 65  wherein the filler fluid comprises one or more alkanes. 
     
     
         70 . The droplet microactuator of  claim 65  wherein the filler fluid comprises a component selected from the group consisting of decane, undecane, dodecane, tridecane, tetradecane, pentadecane, hexadecane. 
     
     
         71 . The droplet microactuator of  claim 65  wherein the filler fluid comprises a component selected from the group consisting of aromatic alkanes. 
     
     
         72 . The droplet microactuator of  claim 65  wherein the filler fluid comprises a mineral oil. 
     
     
         73 . The droplet microactuator of  claim 65  wherein the filler fluid comprises a paraffin oil. 
     
     
         74 . The droplet microactuator of  claim 65  wherein the filler fluid is solid at room temperature of about 25° C. 
     
     
         75 . The droplet microactuator of  claim 65  further comprising a second filler fluid surrounding or in contact with the filler fluid. 
     
     
         76 . The droplet microactuator of  claim 65  wherein the HLB is less than about 10. 
     
     
         77 . The droplet microactuator of  claim 65  wherein the HLB is less than about 5. 
     
     
         78 . The droplet microactuator of  claim 65  wherein the droplet comprises whole blood and the surfactant has an HLB which is less than about 2. 
     
     
         79 . The droplet microactuator of  claim 65  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         80 . The droplet microactuator of  claim 65  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         81 . The droplet microactuator of  claim 65  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         82 . The droplet microactuator of  claim 65  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         83 . The droplet microactuator of  claim 65  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         84 . The droplet microactuator of  claim 65  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         85 . A droplet microactuator comprising:
 (a) a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate;   (b) a second substrate spaced from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and the second substrate; and   (c) a droplet and a filler fluid in the interior volume, wherein the droplet comprises whole blood and is surrounded by the filler fluid, and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes, wherein the filler fluid comprises an oil and a surfactant, and wherein the surfactant comprises nonionic low hydrophile-lipophile balanced (HLB) surfactant with an HLB less than about 2.   
     
     
         86 . The droplet microactuator of  claim 85  wherein the oil comprises one or more silicone oils. 
     
     
         87 . The droplet microactuator of  claim 85  wherein the filler fluid comprises one or more fluorosilicone oils. 
     
     
         88 . The droplet microactuator of  claim 85  wherein the filler fluid comprises one or more hydrocarbons. 
     
     
         89 . The droplet microactuator of  claim 85  wherein the filler fluid comprises one or more alkanes. 
     
     
         90 . The droplet microactuator of  claim 85  wherein the filler fluid comprises a component selected from the group consisting of decane, undecane, dodecane, tridecane, tetradecane, pentadecane, hexadecane. 
     
     
         91 . The droplet microactuator of  claim 85  wherein the filler fluid comprises a component selected from the group consisting of aromatic alkanes. 
     
     
         92 . The droplet microactuator of  claim 85  wherein the filler fluid comprises a mineral oil. 
     
     
         93 . The droplet microactuator of  claim 85  wherein the filler fluid comprises a paraffin oil. 
     
     
         94 . The droplet microactuator of  claim 85  wherein the filler fluid is solid at room temperature of about 25° C. 
     
     
         95 . The droplet microactuator of  claim 85  further comprising a second filler fluid surrounding or in contact with the filler fluid. 
     
     
         96 . The droplet microactuator of  claim 85  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         97 . The droplet microactuator of  claim 85  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         98 . The droplet microactuator of  claim 85  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         99 . The droplet microactuator of  claim 85  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         100 . The droplet microactuator of  claim 85  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         101 . The droplet microactuator of  claim 85  wherein the filler fluid comprises silicone oil and the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w. 
     
     
         102 . A droplet microactuator comprising:
 (a) a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate;   (b) a second substrate spaced from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and the second substrate; and   (c) a droplet and a filler fluid in the interior volume, wherein the droplet comprises whole blood and is surrounded by the filler fluid, and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes, wherein the filler fluid comprises an oil and a surfactant, and wherein the oil comprises a silicone oil and the surfactant comprises a nonionic lipophile surfactant.   
     
     
         103 . The droplet microactuator of  claim 102  wherein the filler fluid comprises one or more fluorosilicone oils. 
     
     
         104 . The droplet microactuator of  claim 102  wherein the filler fluid is solid at room temperature of about 25° C. 
     
     
         105 . The droplet microactuator of  claim 102  further comprising a second filler fluid surrounding or in contact with the filler fluid. 
     
     
         106 . The droplet microactuator of claim  113  wherein the droplet comprises whole blood. 
     
     
         107 . The droplet microactuator of  claim 102  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 10% w/w. 
     
     
         108 . The droplet microactuator of  claim 102  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 1% w/w. 
     
     
         109 . The droplet microactuator of  claim 102  wherein the surfactant is provided in an amount which ranges from about 0.001 to about 0.1% w/w.

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