Fluorescent nanoparticle composites themselves, process for the preparation of such composites, and use in rapid diagnosis systems with affinity to biological molecules

Abstract

The present invention provides fluorescent nanoparticle composites themselves, the process of preparing such composites, to systems for rapid diagnosis (as “kits”) containing such composites, and to the use of such composites. In a preferential embodiment, the composites of the present invention have an affinity for biological molecules, such as DNA. The present invention also comprises the preparation of probes containing biological material, upon which are added fluorescent nanoparticle composites, making viable a rapid and economic biological diagnosis of, for example, diseases and genetic traits, notably in the medical and veterinarian fields. There is yet the fact that the absorption of radiation in the ultraviolet and visible regions provides advantageous use of its fluorescent properties in photovoltaic or electroluminescent devices, such as organic LEDs, or for the increase in luminous gain of fluorescent lamps, which represents another characteristic of the invention.

1 - 26 . (canceled) 
     
     
         27 . Fluorescent nanoparticle composites themselves characterized by comprising:
 a) at least one oxidizing agent;   b) at least one stabilizer agent; and   c) at least one monomer.   
     
     
         28 . The composites, according to  claim 27 , characterized by the fact that said oxidizing agent is a salt in which the cation is selected from the group comprising metals chosen from groups 1B to 8B of the periodic table. 
     
     
         29 . The composites, according to  claim 27 , characterized by the fact that said oxidizing agent is HAuCl 4 . 
     
     
         30 . The composites, according to  claim 27 , characterized m that the stabilizer agent is a compound comprising a mercapto and/or silane group. 
     
     
         31 . The composites, according to  claim 27 , characterized in that the stabilizer is 3-mercaptopropyl-trimethoxy-silane. 
     
     
         32 . The composites, according to  claim 27 , characterized in that the monomer is aniline. 
     
     
         33 . The composites, according to  claim 27 , characterized in that said composites comprise polymeric chains with metallic nanoparticles having about 15 nm or less, used alone or together with magnetic metals or metallic oxides nanoparticles having about 50 nm or less. 
     
     
         34 - 41 . (canceled) 
     
     
         42 . A rapid biological diagnosis kit characterized by the fact that it comprises:
 a) at least one fluorescent composite;   b) at least one short nucleotide sequence;   c) an appropriate substrate or the immobilization of the referred sequence;   d) genetic sample of the patient;   
     
     
         43 . The kit, according to  claim 42 , characterized by the fact that said nucleotide sequence is RNA or single-stranded DNA. 
     
     
         44 . The kit, according to  claim 42 , characterized by the fact that said substrate is a glass slide, paper and/or polymer strip. 
     
     
         45 . A process for fast biological diagnosis characterized by comprising:
 a) immobilizing a short single strand of the nucleotide sequence that uniquely characterizes the organism to be investigated disposed on at least one appropriate support;   b) establishing physical contact among the immobilized material of a), the genetic material obtained from the patient and the composite of fluorescent particles;   c) determining the corresponding fluorescence emission signal.   
     
     
         46 . A method for preparation of diagnostic reactive materials, comprising:
 producing a fluorescent composite by combining at least one oxidizing agent, at least one stabilizer agent and at least one monomer.   
     
     
         47 . A method for preparation of fluorescent polymers, comprising:
 producing a fluorescent composite by combining at least one oxidizing agent, at least one stabilizer agent and at least one monomer.   
     
     
         48 . A method for preparation of fluorescent polymers that contribute to the efficiency increase of photovoltaic or electroluminescent devices through a process of energy transfer between the nanocomposites and active material of the devices, comprising:
 producing a fluorescent composite by combining at least one oxidizing agent, at least one stabilizer agent and at least one monomer.   
     
     
         49 . The method, according to  claim 48 , characterized by the fact that the device is a solar cell or an organic LED. 
     
     
         50 . The method, according to  claim 48 , characterized by the fact that the device is a fluorescent lamp or illumination system. 
     
     
         51 . A method for preparation of a fluorescent test for rapid detection of biological weapons or bioterrorism attacks, comprising:
 producing a fluorescent composite by combining at least one oxidizing agent, at least one stabilizer agent and at least one monomer.