Functionalized surgical adhesives

Abstract

A bioadherent composition includes a first mixture containing a plurality of reactive members of a specific binding pair, said reactive members being bound to a ligand capable of binding a receptor on biological tissue, and a second mixture containing a plurality of complementary reactive members of the specific binding pair, said complementary reactive members being bound to a ligand capable of binding a receptor on biological tissue, said reactive members capable of forming covalent bonds with said complementary reactive members via a reaction selected from Huisgen cycloaddition reactions, Diels-Alder reactions, and/or thiol-alkene reactions. A method for bonding biological tissue involves utilizing the bioadherent composition.

1 - 45 . (canceled) 
     
     
         46 . A bioadherent composition which comprises:
 a first mixture containing a plurality of reactive members of a specific binding pair, said reactive members being bound to a first ligand capable of binding a first receptor on biological tissue; and   a second mixture containing a plurality of complementary reactive members of the specific binding pair, said complementary reactive members being bound to a second ligand capable of binding a second receptor on biological tissue, said reactive members capable of forming covalent bonds with said complementary reactive members via a reaction selected from the group consisting of Huisgen cycloaddition reaction, a Diels-Alder reaction and a thiol-ene reaction.   
     
     
         47 . The bioadherent composition according to  claim 46  wherein the members of the specific binding pair bind to one another via a Huisgen cycloaddition reaction, a Diels-Alder reaction and a thiol-ene reaction. 
     
     
         48 . The bioadherent composition according to  claim 46  wherein the members of the specific binding pair are alkynes and azides. 
     
     
         49 . The bioadherent composition according to  claim 48  wherein the reactive member is an alkyne and the complementary reactive member is an azide. 
     
     
         50 . The bioadherent composition according to  claim 48  wherein the reactive members is an azide and the complementary reactive member is an alkyne. 
     
     
         51 . The bioadherent composition according to  claim 46  wherein the reaction is catalyzed by copper to activate an alkyne and an azide for [3+2] cycloaddition. 
     
     
         52 . The bioadherent composition according to  claim 46  wherein the reaction involves a cyclooctyne reagent and an azide for [3+2] cycloaddition. 
     
     
         53 . The bioadherent composition according to  claim 46  wherein the members of the specific binding pair are thiols and alkenes. 
     
     
         54 . The bioadherent composition according to  claim 46  wherein the members of the specific binding pair are dienes and alkenes. 
     
     
         55 . The bioadherent composition according to  claim 46  wherein the first ligand is an RGD linker. 
     
     
         56 . The bioadherent composition according to  claim 55  wherein the second ligand is an RGD linker. 
     
     
         57 . The bioadherent composition according to  claim 46  wherein the first ligand is selected from the group consisting of antibody, Fab, F(ab′) 2 , Fv, single chain antibody (SCA) and single complementary-determining region (CDR). 
     
     
         58 . The bioadherent composition according to  claim 57  wherein the second ligand is selected from the group consisting of antibody, Fab, F(ab′) 2 , Fv, single chain antibody (SCA) and single complementary-determining region (CDR). 
     
     
         59 . The bioadherent composition according to  claim 46  wherein the first receptor is selected from the group consisting of peptides, oligosaccharides, oligonucleotides and lipids. 
     
     
         60 . The bioadherent composition according to  claim 59  wherein the second receptor is selected from the group consisting of peptides, oligosaccharides, oligonucleotides and lipids. 
     
     
         61 . The bioadherent composition according to  claim 46  wherein the first ligand is capable of bonding to the first receptor on biological tissue to form a bond which is degradable by hydrolysis or enzymatic action. 
     
     
         62 . The bioadherent composition according to  claim 61  wherein the second ligand is capable of bonding to the second receptor on biological tissue to form a bond which is degradable by hydrolysis or enzymatic action.