Strains of lactobacillus with antifungal properties

Abstract

The present invention relates to novel strains of Lactobacillus as well as preparations and compositions, such as fermentation broths, protective cultures, final food or feed product with Lactobacillus alone or in combination with bacteria of the genus Propionibacterium . The present invention further relates to methods for the controlling of growth of a contaminant, such as a bacteria, yeast or mould by using these novel strains of Lactobacillus.

1 . A bacterium of the genus  Lactobacillus  selected from the list consisting of:
 a.  Lactobacillus paracasei  DSM 25832   b.  Lactobacillus plantarum  DSM 25833   c.  Lactobacillus plantarum  DSM 25834   d.  Lactobacillus plantarum  DSM 25835   e.  Lactobacillus plantarum  DSM 25836   f.  Lactobacillus plantarum  DSM 25837;   
       or functional equivalents thereof. 
     
     
         2 . A bacterial preparation, characterized in that it comprises a  Lactobacillus  selected from the list consisting of:
 a.  Lactobacillus paracasei  DSM 25832   b.  Lactobacillus plantarum  DSM 25833   c.  Lactobacillus plantarum  DSM 25834   d.  Lactobacillus plantarum  DSM 25835   e.  Lactobacillus plantarum  DSM 25836   f.  Lactobacillus plantarum  DSM 25837;   
       or functional equivalents thereof, alone or in combination with a bacterium of the genus  Propionibacterium , with any other strain of the genus  Lactobacillus , or with both. 
     
     
         3 . A bacterial preparation according to  claim 2 , which is a cell suspension in fermentation broth, alone or in combination with a bacterium of the genus  Propionibacterium , with another strain of  Lactobacillus , or with both. 
     
     
         4 . A bacterial preparation according to  claim 2 , characterized in that it comprises a strain of  Propionibacterium  selected from  Propionibacterium  acidipropionici DSM 25845 ; Propionibacterium freudenreichii  DSM 25846 ; Propionibacterium  freudenreichii DSM 25847 ; Propionibacterium thoenii  DSM 25848; and  Propionibacterium  thoenii DSM 25849, and  Propionibacterium freudenreichii  subsp.  shermanii  DSM 7067, or a combination of any thereof. 
     
     
         5 . A bacterial preparation according to  claim 2 , which preparation is concentrated. 
     
     
         6 . A bacterial preparation according to  claim 2 , which preparation is freeze-dried. 
     
     
         7 . A bacterial preparation according to  claim 2 , characterized in that it additionally comprises conventional agents used for yeast and mould control. 
     
     
         8 . A method of preparing a final food or feed product comprising adding bacteria of the genus  Lactobacillus  selected from the list consisting of:
 a.  Lactobacillus paracasei  DSM 25832   b.  Lactobacillus plantarum  DSM 25833   c.  Lactobacillus plantarum  DSM 25834   d.  Lactobacillus plantarum  DSM 25835   e.  Lactobacillus plantarum  DSM 25836   f.  Lactobacillus plantarum  DSM 25837;   
       or functional equivalents thereof, to a food or feed product. 
     
     
         9 . (canceled) 
     
     
         10 . A method of controlling the growth of a contaminant characterized by using a bacterial preparation comprising a  Lactobacillus  selected from the list consisting of:
 a.  Lactobacillus paracasei  DSM 25832   b.  Lactobacillus plantarum  DSM 25833   c.  Lactobacillus plantarum  DSM 25834   d.  Lactobacillus plantarum  DSM 25835   e.  Lactobacillus plantarum  DSM 25836   f.  Lactobacillus plantarum  DSM 25837;   
       or functional equivalents thereof, alone or in combination with a bacterium of the genus  Propionibacterium , with another strain of the genus  Lactobacillus , or with both. 
     
     
         11 . (canceled) 
     
     
         12 . A method for controlling the growth of a contaminant in a composition, the method characterized by having in said composition the presence of a viable  Lactibacillus  bacteria of a strain selected from the group consisting of:
 a.  Lactobacillus paracasei  DSM 25832   b.  Lactobacillus plantarum  DSM 25833   c.  Lactobacillus plantarum  DSM 25834   d.  Lactobacillus plantarum  DSM 25835   e.  Lactobacillus plantarum  DSM 25836   f.  Lactobacillus plantarum  DSM 25837;   
       or functional equivalents thereof. 
     
     
         13 . A bacterial preparation according to  claim 2 , characterized in that it additionally comprises propionate or phenylalanine.