Enzymatic Prevention and Control of Biofilm

Abstract

The invention provides methods, compositions, and kits for removal of biofilms from surfaces. The methods described herein comprise simultaneous or sequential application of a perhydrolase enzyme and a mixture of other enzymes, such as proteases, glucanases, esterases, mannanases, phospholipases, cellulases, and/or amylases, to a biofilm on a surface, to effect removal of the biofilm.

1 . A method for removing biofilm from a surface, said method comprising applying a perhydrolase enzyme and a removal enzyme mixture simultaneously to the biofilm for a time sufficient to reduce the biofilm by at least 25%,
 wherein the removal enzyme mixture comprises a protease, a glucanase, and a mannanase.   
     
     
         2 - 4 . (canceled) 
     
     
         5 . A method according to  claim 1 , wherein perhydrolase enzyme and the removal enzyme mixture act synergistically to remove the biofilm from the surface. 
     
     
         6 . A method according to  claim 1 , wherein the biofilm comprises  Pseudomonas aeruginosa, Listeria monocytogenes , or  Staphylococcus aureus.    
     
     
         7 - 8 . (canceled) 
     
     
         9 . A method according to  claim 1 , wherein the perhydrolase enzyme comprises the amino acid sequence set forth in SEQ ID NO:1. 
     
     
         10 - 19 . (canceled)