Novel formulations of pharmacological agents, methods for the preparation thereof and methods for the use thereof

Abstract

In accordance with the present invention, there are provided compositions and methods useful for the in vivo delivery of substantially water insoluble pharmacologically active agents (such as the anticancer drug paclitaxel) in which the pharmacologically active agent is delivered in the form of suspended particles coated with protein (which acts as a stabilizing agent). In particular, protein and pharmacologically active agent in a biocompatible dispersing medium are subjected to high shear, in the absence of any conventional surfactants, and also in the absence of any polymeric core material for the particles. The procedure yields particles with a diameter of less than about 1 micron. The use of specific composition and preparation conditions (e.g., addition of a polar solvent to the organic phase), and careful selection of the proper organic phase and phase fraction, enables the reproducible production of unusually small nanoparticles of less than 200 nm diameter, which can be sterile-filtered. The particulate system produced according to the invention can be converted into a redispersible dry powder comprising nanoparticles of water-insoluble drug coated with a protein, and free protein to which molecules of the pharmacological agent are bound. This results in a unique delivery system, in which part of the pharmacologically active agent is readily bioavailable (in the form of molecules bound to the protein), and part of the agent is present within particles without any polymeric matrix therein.

1 - 65 . (canceled) 
     
     
         66 . A pharmaceutical formulation comprising:
 paclitaxel at a concentration between 5 mg/ml and 15 mg/ml,   wherein the pharmaceutical formulation is an aqueous suspension that is stable for at least 3 days under at least one of room temperature or refrigerated conditions.   
     
     
         67 . The pharmaceutical formulation of  claim 66 , wherein the pharmaceutical formulation is a stable aqueous suspension reconstituted from a sterile lyophilized powder. 
     
     
         68 . The pharmaceutical formulation of  claim 67 , wherein the pharmaceutical formulation comprises paclitaxel at a concentration of 5 mg/ml. 
     
     
         69 . The pharmaceutical formulation of  claim 67 , wherein the pharmaceutical formulation comprises nanoparticles comprising paclitaxel and albumin. 
     
     
         70 . The pharmaceutical formulation of  claim 69 , wherein the average diameter of the nanoparticles is no greater than 220 nm. 
     
     
         71 . The pharmaceutical formulation of  claim 67 , wherein there is substantially no precipitation of paclitaxel for at least 3 days under at least one of room temperature or refrigerated conditions. 
     
     
         72 . The pharmaceutical formulation of  claim 70 , wherein the average nanoparticle size does not substantially change for at least 3 days under at least one of room temperature or refrigerated conditions. 
     
     
         73 . The pharmaceutical formulation of  claim 70 , wherein the nanoparticles comprise paclitaxel and have an albumin coating. 
     
     
         74 . The pharmaceutical formulation of  claim 70 , wherein the nanoparticles have a core and the nanoparticle core is substantially free of polymeric material. 
     
     
         75 . The pharmaceutical formulation of  claim 73 , wherein the albumin coating has free albumin associated therewith, and wherein a portion of the paclitaxel is contained within the albumin coating and a portion of the paclitaxel is associated with the free albumin. 
     
     
         76 . The pharmaceutical formulation of  claim 70 , wherein at least a portion of the albumin is crosslinked by disulfide bonds. 
     
     
         77 . The pharmaceutical formulation of  claim 70 , wherein the paclitaxel is substantially amorphous. 
     
     
         78 . The pharmaceutical formulation of  claim 70 , wherein the paclitaxel is substantially crystalline. 
     
     
         79 . A method, comprising administering an effective amount of the composition of  claim 70  to a patient to treat a tumor. 
     
     
         80 . The method of  claim 79 , wherein the composition is administered parenterally, orally, intravenously, subcutaneously, intraperitoneally, intrathecally, intramuscularly, by inhalation, topically, transdermally, rectally, or vaginally. 
     
     
         81 . The method of  claim 80 , wherein the composition is administered intravenously. 
     
     
         82 . The method of  claim 81 , wherein the pharmaceutical formulation is infused, and the infusion volume is no greater than 200 ml. 
     
     
         83 . A method of treatment, comprising administering an effective amount of the composition of  claim 70  to a patient to treat rheumatoid arthritis. 
     
     
         84 . The method of  claim 83 , wherein the composition is administered parenterally, orally, intravenously, subcutaneously, intraperitoneally, intrathecally, intramuscularly, by inhalation, topically, transdermally, rectally, or vaginally.