Methods and systems for multi-antibody therapies

Abstract

The present invention relates to methods and systems for administering antibody therapeutic agents. The methods include administering one or more (e.g., two or three) binding agents, wherein each of the binding agents has a binding region that is specific to a portion of a disease agent and one or more copies of a tag. The binding agents can be specific to one or more portions of the same or different disease agents. The tag is the same for each of the binding agents. The methods include administering an anti-tag antibody, wherein the anti-tag antibody has an anti-tag region that is specific to the tag, and can have an immunoglobulin (e.g., IgA, IgD, IgE, IgG, and IgM.). Disease agents include bacterial proteins, viral proteins, cancer cells, and proteins or toxins produced therefrom. In particular, the present invention includes methods and systems for binding agents that are specific to neurotoxins that cause botulism.

1 - 8 . (canceled) 
     
     
         9 . A method of treating a bacterial infection, the method comprising:
 a. administering an effective amount of at least one binding agent to a subject having a  Clostridium  bacterial infection and in need thereof, wherein the binding agent comprises a single-chain antibody (scFv) and one or more copies of a tag comprising an antibody epitope, said binding agent having a binding region specific for a portion of a toxin produced by a  Clostridium  bacterium and   b. administering an effective amount of an anti-tag antibody to the subject, wherein the anti-tag antibody has an anti-tag region that is specific for said tag;   wherein:
 the anti-tag antibody binds to one or more copies of the tag of the binding agent and promotes clearance from the subject of the toxin produced by the  Clostridium  bacterium and the binding agent binds to the portion of the toxin produced by the  Clostridium  bacterium. 
   
     
     
         10 . The method of  claim 9 , wherein the administration of the binding agent and the administration of the anti-tag antibody occurs at the same time. 
     
     
         11 . The method of  claim 10 , wherein one or more symptoms associated with the infection with the  Clostridium  bacterium are reduced. 
     
     
         12 - 24 . (canceled) 
     
     
         25 . The method of  claim 9 , wherein the tag comprises an amino acid sequence as shown in SEQ ID NO: 15. 
     
     
         26 . The method of  claim 9 , wherein the administration of the binding agent and the administration of the anti-tag antibody occur at different times.