Methods and devices for separating liquid components

Abstract

Methods and devices for preparing a solid-fibrin web are provided. One method may include drawing blood from a patient, separating plasma from the blood, contacting the plasma with a calcium-coagulation activator and concurrently coagulating and axially centrifuging the plasma to form the solid-fibrin web. The solid-fibrin web may be suitable for regenerating body tissue in a living organism. Devices used in the methods may also be provided.

What is claimed is: 
     
         1 . A device for use in axial centrifugation, the device comprising:
 a container configured for axial centrifugation, the container including
 a primary chamber including an anticoagulant; 
 a secondary chamber containing a coagulator; and 
 a filter separating the primary chamber from the secondary chamber, the filter substantially preventing red and white blood cells, originating from blood drawn into the primary chamber, from entering the secondary chamber under a centrifugal force of about 1000 ×G or greater, but substantially permitting plasma and platelets originating from the blood to flow into the secondary chamber under a centrifugal force of about 1000 ×G or greater. 
   
     
     
         2 . The device of  claim 1 , wherein the primary chamber contains an anti-coagulant. 
     
     
         3 . The device of  claim 1 , wherein the coagulator comprises calcium. 
     
     
         4 . The device of  claim 3 , wherein the coagulator comprises at least one calcium chloride, calcium fluoride, calcium carbonate, calcium gluconate, calcium fumarate, calcium pyruvate, an organic calcium salt and combinations thereof. 
     
     
         5 . A device for use in axial centrifugation, the device comprising:
 a container configured for axial centrifugation, the container including
 a primary chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof; 
 a secondary chamber containing a coagulator; and 
 a diaphragm separating the primary chamber from the secondary chamber, the separation medium substantially preventing fluid communication between the primary and secondary chambers prior to centrifugation, and moving during centrifugation of about 1000 ×G or greater to provide fluid communication between the primary and secondary chambers through the diaphragm. 
   
     
     
         6 . The device of  claim 5 , wherein the separation medium substantially prevents red and white blood cells, originating from blood drawn into the primary chamber, from entering the secondary chamber after centrifugation. 
     
     
         7 . The device of  claim 5 , wherein the primary chamber has a first circumference and the secondary chamber has a second circumference, the first circumference and the second circumference being substantially the same. 
     
     
         8 . The device of  claim 5 , wherein the primary chamber has a first circumference and the secondary chamber has a second circumference, the first circumference being less than the second circumference. 
     
     
         9 . The device of  claim 5 , wherein at least one of the primary chamber and secondary chamber contains a therapeutic enhancing agent. 
     
     
         10 . The device of  claim 9 , wherein the therapeutic enhancing agent comprises at least one of an antibiotic, analgesic, cancer therapeutic, platelet-growth factor, bone morphogenic protein, stem cell, bone graft material, soft tissue graft, platelet-derived growth factor cell culture material, immunosuppressant and a combination thereof. 
     
     
         11 . A device for use in axial centrifugation, the device comprising:
 a container configured for axial centrifugation, the container including
 a first chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof, the first chamber having a first circumference; and 
 a second chamber having a second circumference and containing a coagulator, the second circumference being greater than the first circumference. 
   
     
     
         12 . The device of  claim 11 , wherein the coagulator comprises calcium. 
     
     
         13 . The device of  claim 12 , wherein the activator comprises at least one of calcium chloride, calcium fluoride, calcium carbonate, calcium gluconate, calcium fumarate, calcium pyruvate, an organic calcium salt and combinations thereof. 
     
     
         14 . The device of  claim 11 , wherein the first chamber comprises an upper portion and a lower portion. 
     
     
         15 . The device of  claim 14 , wherein the upper portion is separated from the lower portion by a separator substantially preventing fluid communication therebetween. 
     
     
         16 . The device of  claim 15 , wherein fluid communication is provided between the upper and lower portions when the device is centrifuged at about 1000 ×G or greater. 
     
     
         17 . The device of  claim 16 , wherein the lower portion of the first chamber is in fluid communication with the second chamber. 
     
     
         18 . The device of  claim 11 , further comprising a therapeutic agent. 
     
     
         19 . The device of  claim 11 , wherein the first chamber contains an anti-coagulant.