Method and kit for isolating target cell

Abstract

A particle comprising at least one polymer having negative or positive charges and at least one antibody bound to the polymer, wherein the antibody specifically binds to a surface marker of at least one target cell, and kit comprising same.

1 . A particle comprising at least one polymer having negative or positive charges and at least one antibody bound to the polymer, wherein the antibody specifically binds to a surface marker of at least one target cell. 
     
     
         2 . The particle of  claim 1 , wherein the at least one polymer has negative charges, and the particle is bound to at least one second particle comprising at least one polymer having positive charges, or at least one second particle comprising at least one polymer having positive charges and at least one antibody bound to the polymer of the second particle. 
     
     
         3 . The particle of  claim 1 , wherein the at least one polymer has positive charges, and the particle is bound to at least one second particle comprising at least one polymer having negative charges, or at least one second particle comprising at least one polymer having negative charges and at least one antibody bound to the polymer of the second particle. 
     
     
         4 . The particle of  claim 1 , wherein the charges are negative, and the polymer is polystyrenesulfonate, polyacrylic acid, polymethacrylic acid, polyalcohol, polyphosphate, polymaleic acid, hyaluronic acid, or a combination thereof. 
     
     
         5 . The particle of  claim 1 , wherein the charges are positive, and the polymer is polyaniline, polypyrrol, polyethyleneimine, polylysine, chitosan, or a combination thereof. 
     
     
         6 . The particle of  claim 1 , wherein the particle further comprises a protein which links the polymer to the antibody, and the protein is protein G, protein L, protein A, protein LA, protein AG, or a combination thereof. 
     
     
         7 . The particle of  claim 1 , wherein the particle is a polystyrene particle, latex particle, metal particle, glass particle, magnetic particle, or a combination thereof. 
     
     
         8 . The particle of claim I, wherein the target cell is a circulating tumor cell, cancer stem cell, immunocyte, fetal stem cell, fetal cell, cancer cell, or tumor cell. 
     
     
         9 . The particle of  claim 1 , wherein the particle has a diameter of about 100 nm to about 5 μm. 
     
     
         10 . A kit for isolating a target cell from a biological sample, the kit comprising: a) a particle of  claim 1  having positive charges and a particle of  claim 1  having negative charges; b) a particle of  claim 1  having negative charges and a particle comprising at least one polymer having positive charges; or c) a particle of  claim 1  having positive charges and a particle comprising at least one polymer having negative charges. 
     
     
         11 . The kit of  claim 10 , wherein the kit further comprises a filter having pores, optionally having a diameter of about 1 μm to about 100 μm, about 3 μm to about 50 μm, or about 8 μm to about 30 μm.