Regulatory polynucleotides and uses thereof

Abstract

The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants. Methods for expressing polynucleotides in a plant cell, plant tissue, or plants using the regulatory polynucleotide molecules disclosed herein are also provided.

1 - 40 . (canceled) 
     
     
         41 . A polynucleotide molecule selected from the group consisting of:
 (a) a polynucleotide molecule comprising SEQ ID NO: 148 that is capable of regulating transcription of an operably linked transcribable polynucleotide molecule;   (b) a polynucleotide molecule comprising a sequence having at least about 70% sequence identity to SEQ ID NO: 148 that is capable of regulating transcription of an operably linked transcribable polynucleotide molecule; and   (c) a fragment of the polynucleotide molecule of (a) or (b) capable of regulating transcription of an operably linked transcribable polynucleotide molecule;   wherein the polynucleotide molecule is operably linked to a heterologous transcribable polynucleotide molecule.   
     
     
         42 . The polynucleotide molecule of  claim 41 , wherein the polynucleotide molecule is (a) a polynucleotide molecule comprising SEQ ID NO: 148 that is capable of regulating transcription of an operably linked transcribable polynucleotide molecule. 
     
     
         43 . The polynucleotide molecule of  claim 42 , wherein the polynucleotide molecule is capable of regulating constitutive transcription. 
     
     
         44 . The polynucleotide molecule of  claim 41 , wherein the polynucleotide molecule is (b) a polynucleotide molecule comprising a sequence having at least about 70% sequence identity to SEQ ID NO: 148 that is capable of regulating transcription of an operably linked transcribable polynucleotide molecule. 
     
     
         45 . The polynucleotide molecule of  claim 44 , wherein the polynucleotide molecule is capable of regulating constitutive transcription. 
     
     
         46 . The polynucleotide molecule of  claim 44 , wherein the polynucleotide molecule comprises a sequence having at least about 90% sequence identity to SEQ ID NO: 148. 
     
     
         47 . The polynucleotide molecule of  claim 46 , wherein the polynucleotide molecule comprises a sequence having at least about 95% sequence identity to SEQ ID NO: 148. 
     
     
         48 . The polynucleotide molecule of  claim 47 , wherein the polynucleotide molecule comprises a sequence having at least about 97% sequence identity to SEQ ID NO: 148. 
     
     
         49 . The polynucleotide molecule of  claim 48 , wherein the polynucleotide molecule comprises a sequence having at least about 98% sequence identity to SEQ ID NO: 148. 
     
     
         50 . The polynucleotide molecule of  claim 49 , wherein the polynucleotide molecule comprises a sequence having at least about 99% sequence identity to SEQ ID NO: 148. 
     
     
         51 . The polynucleotide molecule of  claim 41 , wherein the polynucleotide molecule is (c) a fragment of the polynucleotide molecule of (a) or (b) capable of regulating transcription of an operably linked transcribable polynucleotide molecule. 
     
     
         52 . The polynucleotide molecule of  claim 51 , wherein the polynucleotide molecule comprises an intron. 
     
     
         53 . The polynucleotide molecule of  claim 51 , wherein the polynucleotide molecule is capable of regulating constitutive transcription. 
     
     
         54 . The polynucleotide molecule of  claim 41 , wherein the heterologous transcribable polynucleotide molecule encodes a protein of agronomic interest. 
     
     
         55 . The polynucleotide molecule of  claim 41 , wherein the heterologous transcribable polynucleotide molecule is operably linked to a 3′ transcription termination polynucleotide molecule. 
     
     
         56 . A transgenic host cell comprising the polynucleotide molecule of  claim 41 . 
     
     
         57 . The transgenic host cell of  claim 56 , wherein the host cell is a plant cell. 
     
     
         58 . A transgenic plant stably transformed with the polynucleotide molecule of  claim 41 . 
     
     
         59 . The transgenic plant of  claim 58 , wherein the plant is selected from the group consisting of a monocotyledonous and a dicotyledonous plant. 
     
     
         60 . The transgenic plant of  claim 59 , wherein the plant is a monocotyledonous plant selected from the group consisting of wheat, corn, rice, turf grass, millet, sorghum, switchgrass, miscanthus, sugarcane, and Brachypodium. 
     
     
         61 . The transgenic plant of  claim 59 , wherein the plant is a dicotyledonous plant selected from the group consisting of soybean, cotton, canola, and potato. 
     
     
         62 . Seed produced by the transgenic plant of  claim 58 . 
     
     
         63 . A method of directing expression of a transcribable polynucleotide molecule in a host cell comprising:
 (a) introducing the polynucleotide molecule of  claim 41  into a host cell to produce a transgenic host cell; and   (b) selecting a transgenic host cell exhibiting expression of the transcribable polynucleotide molecule.   
     
     
         64 . The method of  claim 63 , wherein the heterologous transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA. 
     
     
         65 . The method of  claim 63 , wherein the host cell is a plant cell. 
     
     
         66 . The method of  claim 65 , further comprising regenerating a plant comprising the introduced polynucleotide molecule from the cell. 
     
     
         67 . A method of directing expression of a transcribable polynucleotide molecule in a plant comprising:
 (a) introducing the polynucleotide molecule of  claim 41  into a plant cell;   (b) regenerating a plant from the plant cell; and   (c) selecting a transgenic plant exhibiting expression of the heterologous transcribable polynucleotide molecule.   
     
     
         68 . The method of  claim 67 , wherein the heterologous transcribable polynucleotide molecule is selected from the group consisting of a coding sequence and a functional RNA.