Methods and assays for oversulfated glycosaminoglycans

Abstract

Methods and assays for oversulfated glycosaminoglycans are provided. In an embodiment, the present disclosure provides a method for detecting oversulfated glycosaminoglycan (OS-GAG) in a heparin sample. The method comprises placing the heparin sample onto a support comprising immobilized heparin and contacting the heparin sample on the support with a binding compound that attaches to the heparin and forms a heparin-binding compound complex. The binding compound also has a greater affinity for attaching to the OS-GAG than to the heparin in the heparin sample and forms an OS-GAG-binding compound complex. The method can further comprise detecting an amount of the heparin-binding compound complex on the support, and determining an amount of OS-GAG in the heparin sample based on the amount of the heparin-binding compound complex on the support.

What is claimed is: 
     
         1 . An assay comprising:
 a support comprising immobilized oversulfated glycosaminoglycan (OS-GAG); and   a binding compound that attaches to the OS-GAG and forms an OS-GAG-binding compound complex.   
     
     
         2 . The assay of  claim 1 , wherein the support is selected from the group consisting of a microtiter plate, a microassay chip and combinations thereof. 
     
     
         3 . The assay of  claim 1 , wherein the binding compound comprises a heparin binding unit. 
     
     
         4 . The assay of  claim 1 , wherein the binding compound is an HB3 construct. 
     
     
         5 . The assay of  claim 4 , wherein the HB3 construct is bound to a component selected from the group consisting of an enzyme, an antibody and combinations thereof. 
     
     
         6 . The assay of  claim 5 , wherein the HB3 construct is labeled with a component selected from the group consisting of a fluorescent molecule, a radionuclide, biotin and combinations thereof. 
     
     
         7 . A compound comprising an oversulfated glycosaminoglycan-binding compound complex formed by a binding compound attached to an oversulfated glycosaminoglycan. 
     
     
         8 . The compound of  claim 7 , wherein the binding compound comprises a heparin binding unit. 
     
     
         9 . The compound of  claim 7 , wherein the binding compound is an HB3 construct. 
     
     
         10 . The compound of  claim 9 , wherein the HB3 construct is bound to a component selected from the group consisting of an enzyme, an antibody and combinations thereof. 
     
     
         11 . The compound of  claim 7 , wherein the HB3 construct is labeled with a component selected from the group consisting of a fluorescent molecule, a radionuclide, biotin and combinations thereof.