Universal sample preparation system and use in an integrated analysis system

Abstract

The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

1 . A device comprising:
 a microfluidic microchip comprising at least one port aperture fluidically connected to at least one microfluidic channel in the microfluidic microchip, wherein the channel comprises at least one valve that controls movement of a fluid through the channel; and   a cartridge mated to the microchip and comprising a chamber,
 wherein said chamber comprises two chamber apertures that are each aligned with a port aperture of said microfluidic microchip. 
   
     
     
         2 .- 18 . (canceled) 
     
     
         19 . The device of  claim 1 , wherein said cartridge further comprises at least one input port,
 wherein said at least one input port is adapted to mate with a delivery device,   wherein said delivery device is fluidically connected to the fluidic layer of said microfluidic microchip; and   wherein one of said at least one chamber is a closed reaction chamber fluidically connected to the fluidic layer of said microfluidic microchip.   
     
     
         20 .- 28 . (canceled) 
     
     
         29 . A method for performing biochemical reactions comprising:
 (a) providing the device of  claim 1 , and   (b) performing at least one enzymatic reaction within said chamber.   
     
     
         30 .- 31 . (canceled) 
     
     
         32 . A method for enriching at least one component from a sample comprising:
 (a) mating a delivery device to said input port of the device of claim  2 ,   (b) treating said sample with at least one reagent to increase the availability of said at least one component for enrichment,   (c) delivering said at least one component to said at least one reaction chamber of said cartridge,   (d) binding said component to one or more particles in said at least one closed reaction chamber,   (e) washing said particle bound component to remove waste, and   (f) eluting said particle bound component.   
     
     
         33 .- 94 . (canceled)