Prebiotic and preservative uses of oil emulsified probiotic encapsulations

Abstract

An encapsulation system is provided comprising nitrogen-purge, instant bonding encapsulation method. Specifically, the encapsulation system comprises a composition, a two-piece capsule comprising a capsule cap and a capsule body; a gas to purge oxygen from the composition within the capsule; and a sealing solution to seal the capsule cap to the capsule body. Associated methods for encapsulating compositions using the encapsulation system are also provided.

I claim: 
     
         1 . A probiotic composition comprising: a Lactic Acid Bacteria (LAB) suspended in an edible oil wherein said edible oil has prebiotic properties 
     
     
         2 . The probiotic composition according to  claim 1  wherein said LAB is selected from the group consisting of  Lactobacillus acidophilus, L. amylovorus, L. brevis, L. bulgaricus, L. casei  spp.  casei, L. casei  spp.  rhamnosus, L. crispatus, L. delbrueckii  ssp.  lactis, L. fermentum, L. helvaticus, L. johnsonii, L. paracasei, L. pentosus, L. plantarum, L. reuteri, L. sake, Bifidobacterium animalis, B. bifidum, B. breve, B. infantis, B. longum, Pediococcus acidilactici, Propionibacterium acidipropionici, P. freudenreichii, P. jensenii, P. theonii, Streptococcus cremoris, S. lactis, S. thermophilus  and combinations thereof. 
     
     
         3 . The probiotic composition according to  claim 1  wherein said prebiotic edible oil is selected from the group consisting of fish oil, olive oil, rice-bran oil, soy oil and combinations thereof. 
     
     
         4 . The probiotic composition according to  claim 1  wherein said LAB is  L. casei  strain KE01 having ATCC number PTA 3945. 
     
     
         5 . A pair of KE01 specific primers wherein said KE01 specific primers consists of positive and negative strand and said positive primer is SEQ ID NO 1 and said negative strand is SEQ ID NO 2.