Potential Regenerative Cell and Its Culturing Method

Abstract

The present invention relates to a type of cell—potential regenerative cell (PRC) capable of continuous proliferation, and generated mammal (including human) cells, tissues and tissue-organs by in vitro culture and replication of PRCs. The present invention also relates to the methods and cell growth regulators for culturing mammal (including human) PRCs, tissues, and tissue-organs.

What is claimed is: 
     
         1 . A cell mixture including activated mammalian (including human) potential regenerative cells (PRCs) capable of continuous proliferation, wherein said PRCs have proliferative potential of stem cells but exist in a tissue in a morphology of normal tissue cells, wherein in vitro culture of said PRCs provides proliferative cells, and said cell mixture is obtained by the following methods:
 obtaining mammalian (including human) adult tissue cells and/or tissue;   culturing the said mammalian (including human) adult tissue cells and/or tissue in a medium to activate the PRCs;   culturing the activated PRCs to proliferate continuously and exhibit stem cell characteristics;   wherein a cell growth regulator is added in the medium, said cell growth regulator contains at least sterols dissolved in oil and baicalin at a concentration of 0.1%-2% by weight based on the total weight of the regulator.   
     
     
         2 . The cell mixture according to  claim 1 , wherein said activated PRCs proliferate and differentiate, in vitro, into cells having a same origin as said PRCs. 
     
     
         3 . The cell mixture according to  claim 1 , wherein said activated PRCs proliferate and differentiate, in vitro, into cells having a different origin as said PRCs. 
     
     
         4 . The cell mixture according to  claim 1 , wherein said activated PRCs proliferate and differentiate, in vitro, into tissues having a same origin as said PRCs. 
     
     
         5 . The cell mixture according to  claim 1 , wherein said activated PRCs proliferate and differentiate, in vitro, into tissues having a different origin as said PRCs. 
     
     
         6 . The cell mixture according to  claim 1 , wherein said activated PRCs and the generated proliferative cells and tissues by the culturing of said activated PRCs, are capable of being used for in vitro biological models and transplantation therapy. 
     
     
         7 . The cell mixture according to  claim 1 , wherein said activated PRCs are capable of being used as biological models for scientific research and drug active ingredient screening, and for toxicology studies of pharmaceutical and food. 
     
     
         8 . The cell mixture according to  claim 1 , wherein said activated PRCs are capable of being used as biological models for cancer research 
     
     
         9 . The cell mixture according to  claim 1 , wherein said activated PRCs can be used as biological models for research on all substances activating and adapting to life. 
     
     
         10 . A method for culturing a cell mixture including activated mammalian (including human) potential regenerative cells (PRCs), wherein the method comprises the steps of:
 A, obtaining mammalian (including human) adult tissue cells and/or tissue;   B, culturing the said mammalian (including human) adult tissue cells and/or tissue in a medium to activate the PRCs;   C, culturing activated PRCs to proliferate continuously and exhibit stem cell characteristics;   wherein a cell growth regulator is added in the medium, said cell growth regulator contains at least sterols dissolved in oil and baicalin at a concentration of 0.1%-2% by weight based on the total weight of the regulator.   
     
     
         11 . The method according to  claim 10 , wherein said oil is animal oil or vegetable oil, wherein the vegetable oil is at least one selected from corn oil, peanut oil, cottonseed oil, safflower oil, tea tree oil, sesame oil, olive oil and soybean oil. 
     
     
         12 . The method according to  claim 10 , wherein said sterols is comprised of animal sterols or Phytosterols, wherein the animal sterols include cholesterol and all its natural or synthetic isomers and derivatives. 
     
     
         13 . The method according to  claim 12 , wherein said sterols is comprised of at least one of stigmasterol, β-sitosterol, ergosterol, γ-sitosterol, brassicasterol, α-spinasterol, 24-dehydrocholesterol, poriferasterol, daucosterol and all natural or synthetic isomers and derivatives, and combinations thereof. 
     
     
         14 . The method according to  claim 13 , wherein said sterol is a combination of stigmasterol, β-sitosterol and brassicasterol. 
     
     
         15 . The method according to  claim 10 , wherein the amount of the sterol in the cell growth regulator is 0.5%-20% by weight based on the total weight of the regulator. 
     
     
         16 . The method according to  claim 15 , wherein the amount of the sterol in the cell growth regulator is 1%-10% by weight based on the total weight of the regulator. 
     
     
         17 . The method according to  claim 16 , wherein the amount of the sterol in the cell growth regulator is 2%-6% by weight based on the total weight of the regulator. 
     
     
         18 . The method according to  claim 10 , wherein the cell growth regulator comprises also beeswax at a concentration of 1%-20% by weight based on the total weight of the regulator. 
     
     
         19 . The method according to  claim 18 , wherein the concentration of beeswax is 2%-10% by weight based on the total weight of the regulator. 
     
     
         20 . The method according to  claim 19 , wherein the concentration of beeswax is 3%-6% by weight based on the total weight of the regulator. 
     
     
         21 . The method according to  claim 6 , wherein the cell growth regulator comprises also propolis at a concentration of 0.1%-30% by weight based on the total weight of the regulator. 
     
     
         22 . The method according to  claim 19 , wherein the concentration of propolis is 1%-20% by weight based on the total weight of the regulator. 
     
     
         23 . The method according to  claim 22 , wherein the concentration of propolis is 5%-10% by weight based on the total weight of the regulator. 
     
     
         24 . The method according to  claim 10 , wherein the concentration of baicalin is 0.2%-1% by weight based on the total weight of the regulator. 
     
     
         25 . The method according to  claim 10 , wherein the concentration of baicalin is 0.5%-1% by weight based on the total weight of the regulator. 
     
     
         26 . The method according to  claim 10 , wherein the cell growth regulator comprises also obaculactone at a concentration of 0.1%-2% by weight based on the total weight of the regulator. 
     
     
         27 . The method according to  claim 26 , wherein the concentration of obaculactone is 0.2%-1% by weight based on the total weight of the regulator. 
     
     
         28 . The method according to  claim 27 , wherein the concentration of obaculactone is 0.5%-1% by weight based on the total weight of the regulator. 
     
     
         29 . The method according to  claim 10 , wherein the cell growth regulator comprises also yellow berberine at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         30 . The method according to  claim 29 , wherein the concentration of yellow berberine is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         31 . The method according to  claim 30 , wherein the concentration of yellow berberine is 0.003%-0.1% by weight based on the total weight of the regulator. 
     
     
         32 . The method according to  claim 10 , wherein the cell growth regulator comprises also berberine at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         33 . The method according to  claim 32 , wherein the concentration of berberine is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         34 . The method according to  claim 10 , wherein the cell growth regulator comprises also narcotoline at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         35 . The method according to  claim 34 , wherein the concentration of narcotoline is 0.002%-0.5% or  0 . 003 %- 0 . 1 % by weight based on the total weight of the regulator. 
     
     
         36 . The method according to  claim 10 , wherein the cell growth regulator comprises also earthworm at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         37 . The method according to  claim 36 , wherein the concentration of earthworm is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         38 . The method according to  claim 37 , wherein the concentration of earthworm is 0.003%-0.1% by weight based on the total weight of the regulator. 
     
     
         39 . The method according to  claim 10 , wherein the cell growth regulator comprises also yellow berberine, berberine, and narcotoline. 
     
     
         40 . The method according to  claim 10 , wherein the cell growth regulator comprises beeswax, propolis, baicalin, obaculactone, yellow berberine, berberine, narcotoline and earthworm. 
     
     
         41 . The method according to  claim 10 , wherein an amount of the cell growth regulator added into the medium is in an amount of 10 g-50 g per 100 ml medium. 
     
     
         42 . The method according to  claim 41 , wherein an amount of the cell growth regulator added into the medium is in an amount of 20 g-30 g per 100 ml medium. 
     
     
         43 . The method according to  claim 42 , wherein an amount of the cell growth regulator added into the medium is in an amount of 20 g per 100 ml medium. 
     
     
         44 . The method of using a composition to prepare a cell growth regulator, wherein the composition contains at least sterols dissolved in oil and baicalin at a concentration of 0.1%-2% by weight based on the total weight of the regulator. 
     
     
         45 . The method of use according to  claim 44 , wherein said oil is animal oil or vegetable oil, And the vegetable oil is at least one selected from corn oil, peanut oil, cottonseed oil, safflower oil, tea tree oil, sesame oil, olive oil and soybean oil. 
     
     
         46 . The method of use according to  claim 44 , wherein said sterols comprise of animal sterols or phytosterols, wherein the animal sterols include cholesterol and all its natural or synthetic isomers and derivatives. 
     
     
         47 . The method of use according to  claim 44 , wherein said sterols comprise of at least one of stigmasterol, β-sitosterol, ergosterol, γ-sitosterol, brassicasterol, α-spinasterol, 24-dehydrocholesterol, poriferasterol, daucosterol and all natural or synthetic isomers and derivatives, and combinations thereof. 
     
     
         48 . The method of use according to  claim 47 , wherein said sterol is a combination of stigmasterol, β-sitosterol and brassicasterol. 
     
     
         49 . The method of use according to  claim 44 , wherein the amount of the sterol in the cell growth regulator is 0.5%-20% by weight based on the total weight of the regulator. 
     
     
         50 . The method of use according to  claim 45 , wherein the amount of the sterol in the cell growth regulator is 1%-10% by weight based on the total weight of the regulator. 
     
     
         51 . The method of use according to  claim 50 , wherein the amount of the sterol in the cell growth regulator is 2%-6% by weight based on the total weight of the regulator. 
     
     
         52 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also beeswax at a concentration of 1%-20% by weight based on the total weight of the regulator. 
     
     
         53 . The method of use according to  claim 51 , wherein the concentration of beeswax is 2%-10% by weight based on the total weight of the regulator. 
     
     
         54 . The method of use according to  claim 53 , wherein the concentration of beeswax is 3%-6% by weight based on the total weight of the regulator. 
     
     
         55 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also propolis at a concentration of 0.1%-30% by weight based on the total weight of the regulator. 
     
     
         56 . The method of use according to  claim 55 , wherein the concentration of propolis is 1%-20%  by weigh based on the total weight of the regulator. 
     
     
         57 . The method of use according to  claim 56 , wherein the concentration of propolis is 5%-10% by weight based on the total weight of the regulator. 
     
     
         58 . The method of use according to  claim 44 , wherein the concentration of baicalin is 0.2%-1% by weight based on the total weight of the regulator. 
     
     
         59 . The method of use according to  claim 58 , wherein the concentration of baicalin is 0.5%-1% by weight based on the total weight of the regulator. 
     
     
         60 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also obaculactone at a concentration of 0.1%-2% by weight based on the total weight of the regulator. 
     
     
         61 . The method of use according to  claim 60 , wherein the concentration of obaculactone is 0.2%-1% by weight based on the total weight of the regulator. 
     
     
         62 . The method of use according to  claim 60 , wherein the concentration of obaculactone is 0.5%-1% by weight based on the total weight of the regulator. 
     
     
         63 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also yellow berberine at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         64 . The method of use according to  claim 63 , wherein the concentration of yellow berberine is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         65 . The method of use according to  claim 64 , wherein the concentration of yellow berberine is 0.003%-0.1% by weight based on the total weight of the regulator. 
     
     
         66 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also berberine at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         67 . The method of use according to  claim 66 , wherein the concentration of berberine is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         68 . The method of use according to  claim 67 , wherein the concentration of berberine is 0.003%-0.1% by weight based on the total weight of the regulator. 
     
     
         69 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also narcotoline at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         70 . The method of use according to  claim 44 , wherein the cell growth regulator comprises also earthworm at a concentration of 0.001%-2% by weight based on the total weight of the regulator. 
     
     
         71 . The method of use according to  claim 70 , wherein the concentration of earthworm is 0.002%-0.5% by weight based on the total weight of the regulator. 
     
     
         72 . The method of use according to  claim 71 , wherein the concentration of earthworm is 0.003%-0.1% by weight based on the total weight of the regulator. 
     
     
         73 . The method of use according to  claim 44 , wherein the cell growth regulator comprises any combination composed of at least one substance selected from the group of beeswax, propolis, baicalin, obaculactone, yellow berberine, berberine, narcotoline and earthworm. 
     
     
         74 . The method of use according to any one of  claims 44 - 73 , wherein the cell growth regulator is used for in vitro culture and replication of cells and tissues of plant or mammal (including human). 
     
     
         75 . The method of use according to any one of  claims 44 - 73 , wherein the culture medium contains said cell growth regulator. 
     
     
         76 . The method of use according to  claim 75 , wherein said culture medium is at least one selected from a group which includes: Eagle MEM culture medium and their derivatives, Ham's F-12 medium, RPMI 1640 medium, 199 medium, L15 medium, Fischer medium, MB752/1 medium, CMRL1066 medium, McCoy5A medium, FBS, newborn calf serum, horse serum, rat tail collagen, hydrolyzed lactoprotein, chicken serum, rabbit serum, and goat serum.