Glucose-induced inactivation/degradation-resistant transporter gene and use thereof

Abstract

The present invention relates to a glucose-induced inactivation/degradation-resistant transporter gene and use thereof, and more particularly, to a brewing yeast having excellent assimilation of oligosaccharides (maltose, maltotriose, etc.), an alcoholic beverage prepared using the yeast, a method of producing the alcoholic beverage, etc. More specifically, the present invention relates to a glucose-induced inactivation/degradation-resistant transporter including Mal21p, mutant Mal31p, mutant Mal61p, mutant Mtt1p, mutant Agt1p, etc., a gene encoding the transporter, a method of producing an alcoholic beverage using thereof, and so on.

What is claimed is: 
     
         1 . An isolated, non-naturally occurring polynucleotide comprising any one of the nucleotide sequences of SEQ ID NOs: 25, 27, 29, 35, 37 39, 41, 43, 45, 47, or 49, or a nucleotide sequence that is at least 90% identical to any one of the nucleotide sequences of SEQ ID NOs: 25, 27, 29, 35, 37, 39, 41, 43, 45, 47, or 49, wherein the polynucleotide encodes a protein having resistance to glucose-induced inactivation or degradation. 
     
     
         2 . The isolated polynucleotide of  claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 25, 27, or 29. 
     
     
         3 . The isolated polynucleotide of  claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 35. 
     
     
         4 . The isolated polynucleotide of  claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 37, 39, 41, 43, 45, 47, or 49. 
     
     
         5 . The isolated polynucleotide of  claim 1 , which is at least 95% identical to any one of the nucleotide sequences of SEQ ID NOs: 25, 27, or 29, and encodes a protein having resistance to glucose-induced inactivation or degradation. 
     
     
         6 . The isolated polynucleotide of  claim 1 , which is at least 95% identical to the nucleotide sequence of SEQ ID NO: 35,and encodes to protein having resistance to glucose induced inactivation or degradation. 
     
     
         7 . The isolated polynucleotide of  claim 1 , which it least 95% identical to any one of the nucleotide sequences of SEQ ID NOs: 37, 39, 41, 43, 45, 47, or 49, and encodes a protein having resistance to glucose-induced inactivation or degradation. 
     
     
         8 . The polynucleotide according to  claim 1 , which is DNA. 
     
     
         9 . A protein encoded by the polynucleotide according to  claim 1 . 
     
     
         10 . A vector comprising the polynucleotide according to  claim 1 . 
     
     
         11 . A transformed yeast introduced with the vector according to  claim 10 . 
     
     
         12 . The yeast according to  claim 11 , wherein oligosaccharide assimilability is improved by increasing the expression level of the isolated polynucleotide. 
     
     
         13 . An isolated polynucleotide that encodes a protein consisting of the amino acid sequence of SEQ ID NO: 26, 28, or 30. 
     
     
         14 . An isolated polynucleotide that encodes a protein consisting of the amino acid sequence of SEQ ID NO: 36. 
     
     
         15 . An isolated polynucleotide that encodes a protein consisting of the amino acid sequence of SEQ ID NO: 38, 40, 42, 44, 46, 48, or 50.