Isoforms of the human sst5 receptor originated by alternative splicing and oligonucleotide pairs to detect them by pcr

Abstract

The present invention is referred to two human nucleic 5 acids that comprise sequences encoding two new isoforms of the human somatostatin receptor type 5 originated by alternative splicing, named sst5B and sst5C, with possible involvement in tumor processes. In addition, the invention is referred to oligonucleotide pairs allowing 10 their differential detection in several tissues using the PCR technique.

1 . A method of selectively amplifying a human somatostatin receptor type 5 nucleic acid, wherein purified oligonucleotides, fragments, homologs, or complementary nucleic acids thereof comprising a sequence having at least 90% homology with SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13 or SEQ ID NO:14, are used for the PCR amplification of a human somatostatin receptor type 5 nucleic acid. 
     
     
         2 . A method of gene silencing the expression of either or both sst5B or sst5C somatostatin receptor type 5 genes, said method comprising administering a somatostatin receptor type 5 nucleic acid, fragment, homolog or complementary nucleic acid comprising a sequence having at least 90% homology to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7.