Method of making a formulation for deactivating nucleic acids

Abstract

The disclosure relates to formulations for use in deactivating nucleic acids and methods of making and using the same.

1 . A composition for deactivating nucleic acids, the composition comprising:
 hydrogen peroxide at a concentration of about 0.5% to about 30% (w/v);   a metal ion source at a concentration of about 0.1 mM to about 5 mM; and   piperazine at a concentration of about 0.5 mM to about 250 mM.   
     
     
         2 . The composition of  claim 1 , wherein the metal ions comprise at least one of copper ions, cobalt ions, iron ions and manganese ions. 
     
     
         3 . The composition of  claim 2 , wherein the metal ions comprise copper ions. 
     
     
         4 . The composition of  claim 3 , wherein the copper ion source is cupric sulfate or cupric acetate. 
     
     
         5 . The composition of  claim 4 , wherein the copper ion source is cupric sulfate. 
     
     
         6 . The composition of  claim 1 , wherein:
 the hydrogen peroxide is at a concentration of about 1% to 15% (w/v);   the metal ion source is at a concentration of about 0.5 mM to about 2.5 mM; and   the piperazine is at a concentration of about 1 mM to about 200 mM.   
     
     
         7 . The composition of  claim 6 , wherein the metal ions comprise at least one of copper ions, cobalt ions, iron ions and manganese ions. 
     
     
         8 . The composition of  claim 7 , wherein the metal ions comprise copper ions. 
     
     
         9 . The composition of  claim 8 , wherein the copper ion source is cupric sulfate or cupric acetate. 
     
     
         10 . The composition of  claim 9 , wherein the copper ion source is cupric sulfate. 
     
     
         11 . The composition of  claim 1 , wherein:
 the hydrogen peroxide is at a concentration of about 1% to about 6% (w/v);   the metal ion source is at a concentration of about 1 mM to about 2.5 mM; and   the piperazine is at a concentration of about 10 mM to about 100 mM.   
     
     
         12 . The composition of  claim 11 , wherein the metal ions comprise at least one of copper ions, cobalt ions, iron ions and manganese ions. 
     
     
         13 . The composition of  claim 12 , wherein the metal ions comprise copper ions. 
     
     
         14 . The composition of  claim 13 , wherein the copper ion source is cupric sulfate or cupric acetate. 
     
     
         15 . The composition of  claim 14 , wherein the copper ion source is cupric sulfate. 
     
     
         16 . The composition of  claim 1 , wherein the composition comprises about 3% (w/v) hydrogen peroxide, about 2 mM cupric sulfate, and about 50 mM piperazine. 
     
     
         17 . The composition of  claim 16 , wherein the pH is about 5.5.