Transformation system in camelina sativa

Abstract

The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium -mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

1 - 29 . (canceled) 
     
     
         30 . A method of transferring a recombinant DNA construct into a  Camelina sativa  plant cell, the method comprising:
 a) contacting one or more plant cells from  Camelina sativa  with  Agrobacterium,  wherein the  Agrobacterium  comprises a recombinant DNA construct;   b) maintaining the one or more plant cells in contact with the  Agrobacterium  under conditions such that  Agrobacterium -mediated genetic transformation of the one or more plant cells occurs, forming one or more plant cells comprising the recombinant DNA construct.   
     
     
         31 . The method of  claim 30 , further comprising producing a plant or plant part comprising the recombinant DNA construct from the one or more plant cells comprising the recombinant DNA construct. 
     
     
         32 . The method of  claim 30 , wherein the  Agrobacterium  is  Agrobacterium tumefaciens.    
     
     
         33 . The method of  claim 30 , wherein the recombinant DNA construct is a vector comprising an optional selectable marker gene. 
     
     
         34 . The method of  claim 33 , wherein the selectable marker gene comprises an antibiotic resistance gene. 
     
     
         35 . The method of  claim 34 , wherein the antibiotic resistance gene comprises a neomycin phosphotransferase (npt ll) gene or a hygromycin phosphotransferase (hpt) gene. 
     
     
         36 . The method of  claim 30 , wherein the  Camelina sativa  plant cell is found in an organ selected from the group consisting of hypocotyl, cotyledon, stem, leaf, and other plant organs. 
     
     
         37 . The method of  claim 30 , wherein the recombinant DNA construct comprises at least one heterologous gene, at least one promoter sequence, and an optional selectable marker gene. 
     
     
         38 . The method of  claim 30 , wherein, in step b), the plant cell is exposed to a growth medium comprising about 2% sucrose. 
     
     
         39 . The method of  claim 38 , wherein the growth media further comprises one or more hormones. 
     
     
         40 . The method of  claim 39 , wherein the one or more hormones are selected from the group consisting of auxin and cytokinin. 
     
     
         41 . The method of  claim 40 , wherein the auxin is NAA (α-napthaleneacetic acid) and the cytokinin is BAP (6-benzylaminopurine). 
     
     
         42 . The method of  claim 30 , wherein the plant cell is maintained in contact with the  Agrobacterium  for at least two days. 
     
     
         43 . The method of  claim 30 , wherein the one or more plant cells comprising the recombinant DNA construct produce a heterologous product. 
     
     
         44 . The method of  claim 43 , wherein the heterologous product is a protein or a metabolite. 
     
     
         45 . A  Camelina sativa  plant cell comprising a recombinant DNA construct, characterized in that said plant cell is obtained by the method according to  claim 30 . 
     
     
         46 . A  Camelina sativa  plant or plant part comprising a recombinant DNA construct, characterized in that said plant or plant part is derived from a plant cell obtained by the method according to  claim 30 .