US2014220180A1PendingUtilityA1

Ferulate esterase producing strains and methods of using same

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Assignee: PIONEER HI BRED INTPriority: Sep 1, 2004Filed: Apr 4, 2014Published: Aug 7, 2014
Est. expirySep 1, 2024(expired)· nominal 20-yr term from priority
Inventors:Carol A. HendrickVictor NserekoWilliam RutherfordBrenda SmileyAnnette SpielbauerBarbara G. Ruser
A23K 10/12C12P 5/023A23K 10/18A23K 30/18A61K 35/74C12N 9/16C12R 2001/225C12R 2001/25C12R 2001/24C12N 1/205Y02E50/30Y02P60/87A23K 1/007
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Claims

Abstract

Ferulate esterase producing bacterial strains or functional mutants thereof and methods of using ferulate esterase producing bacterial strains as forage additives are disclosed.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A substantially purified strain of a bacterium selected from the group consisting of  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 , Lactobacillus plantarum , strain LP678, deposited as Patent Deposit No. PTA-6134,  Lactobacillus plantarum , strain LP3710, deposited as Patent Deposit No. PTA-6136,  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137,  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139,  Lactobacillus paracasei tolerans , strain LC3200 having ATCC Accession No. PTA-6135,  Lactobacillus brevis , strain LB1154, deposited as Patent Deposit NRRL B-30865,  Lactobacillus buchneri , strain LN4888, deposited as Patent Deposit NRRL B-30866,  Lactobacillus reuteri , strain LR4933, deposited as Patent Deposit NRRL B-30867,  Lactobacillus crispatus , strain LI2127, deposited as Patent Deposit NRRL B-30868,  Lactobacillus crispatus , strain LI2350, deposited as Patent Deposit NRRL B-30869,  Lactobacillus crispatus , strain LI2366, deposited as Patent Deposit NRRL B-30870,  Lactobacillus  species unknown, strain UL3050, deposited as Patent Deposit NRRL B-30871, and mixtures thereof. 
     
     
         2 . A biologically pure culture of a bacterium selected from the group consisting of  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138,  Lactobacillus plantarum , strain LP678, deposited as Patent Deposit No. PTA-6134,  Lactobacillus plantarum , strain LP3710, deposited as Patent Deposit No. PTA-6136,  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137,  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139,  Lactobacillus paracasei tolerans , strain LC3200 having ATCC Accession No. PTA-6135,  Lactobacillus brevis, strain LB 1154, deposited as Patent Deposit NRRL B-30865,  Lactobacillus buchneri , strain LN4888, deposited as Patent Deposit NRRL B-30866,  Lactobacillus reuteri , strain LR4933, deposited as Patent Deposit NRRL B-30867,  Lactobacillus crispatus , strain LI2127, deposited as Patent Deposit NRRL B-30868,  Lactobacillus crispatus , strain LI2350, deposited as Patent Deposit NRRL B-30869,  Lactobacillus crispatus , strain LI2366, deposited as Patent Deposit NRRL B-30870,  Lactobacillus  species unknown, strain UL3050, deposited as Patent Deposit NRRL B-30871. 
     
     
         3 . A composition comprising a ferulate esterase producing bacterial strain and a suitable carrier, wherein the bacterial strain is any one of  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 , Lactobacillus plantarum , strain LP678, deposited as Patent Deposit No. PTA-6134 , Lactobacillus plantarum , strain LP3710, deposited as Patent Deposit No. PTA-6136 , Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137 , Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 , Lactobacillus brevis , strain LB1154, deposited as Patent Deposit NRRL B-30865,  Lactobacillus buchneri , strain LN4888, deposited as Patent Deposit NRRL B-30866,  Lactobacillus reuteri , strain LR4933, deposited as Patent Deposit NRRL B-30867,  Lactobacillus crispatus  LI2127, deposited as Patent Deposit NRRL B-30868,  Lactobacillus crispatus , strain LI2350, deposited as Patent Deposit NRRL B-30869,  Lactobacillus crispatus , strain LI2366, deposited as Patent Deposit NRRL B-30870, and mixtures thereof. 
     
     
         4 . The composition of  claim 3 , wherein said composition further comprises  Lactobacillus paracasei tolerans , strain LC3200, deposited as Patent Deposit No. PTA-6135. 
     
     
         5 . The composition of  claim 4 , wherein said ferulate esterase producing bacterial strain comprises  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139. 
     
     
         6 . The composition of  claim 5 , wherein the composition comprises from about 10 1  to about 10 10  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 1  to about 10 10  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         7 . The composition of  claim 5 , wherein the composition comprises from about 10 2  to about 10 7  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 2  to about 10 7  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         8 . The composition of  claim 5 , wherein the composition comprises from about 10 3  to about 10 6  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 3  to about 10 6  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         9 . The composition of  claim 4 , wherein said ferulate esterase producing bacterial strain comprises  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137. 
     
     
         10 . The composition of  claim 9 , wherein the composition comprises from about 10 1  to about 10 10  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 1  to about 10 10  viable organisms of said  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137 per gram of a pre-ensiled plant material. 
     
     
         11 . The composition of  claim 9 , wherein the composition comprises from about 10 2  to about 10 7  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 2  to about 10 7  viable organisms of said  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137 per gram of a pre-ensiled plant material. 
     
     
         12 . The composition of  claim 9 , wherein the composition comprises from about 10 3  to about 10 6  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 3  to about 10 6  viable organisms of said  Lactobacillus plantarum , strain LP3779, deposited as Patent Deposit No. PTA-6137 per gram of a pre-ensiled plant material. 
     
     
         13 . The composition of  claim 4 , wherein said ferulate esterase producing bacterial strain comprises  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138. 
     
     
         14 . The composition of  claim 13 , wherein the composition comprises from about 10 1  to about 10 10  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 1  to about 10 10  viable organisms of said  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 per gram of a pre-ensiled plant material. 
     
     
         15 . The composition of  claim 13 , wherein the composition comprises from about 10 2  to about 10 7  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 2  to about 10 7  viable organisms of said  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 per gram of a pre-ensiled plant material. 
     
     
         16 . The composition of  claim 13 , wherein the composition contains from about 10 3  to about 10 6  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 3  to about 10 6  viable organisms of said  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 per gram of a pre-ensiled plant material. 
     
     
         17 . The composition of  claim 4 , wherein said ferulate esterase producing bacterial strain comprises  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139. 
     
     
         18 . The composition of  claim 17 , wherein the composition comprises from about 10 1  to about 10 10  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 1  to about 10 10  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         19 . The composition of  claim 17 , wherein the composition comprises from about 10 2  to about 10 7  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 2  to about 10 7  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         20 . The composition of  claim 19 , wherein the composition contains from about 10 3  to about 10 6  viable organisms of said  Lactobacillus paracasei tolerans , strain LC3200 per gram of a pre-ensiled plant material and from about 10 3  to about 10 6  viable organisms of said  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139 per gram of a pre-ensiled plant material. 
     
     
         21 . The composition of  claim 4 , wherein said ferulate esterase producing bacterial strain comprises  Lactobacillus buchneri , strain LN4017, deposited as Patent Deposit No. PTA-6138 and  Lactobacillus plantarum , strain LP7109, deposited as Patent Deposit No. PTA-6139. 
     
     
         22 . The composition of  claim 3 , wherein the composition further comprises pre-ensiled plant material. 
     
     
         23 . The composition of  claim 22 , wherein the pre-ensiled plant material is selected from the group consisting of grasses, maize, alfalfa, wheat, legumes, sorghum, sunflower, barley and mixtures thereof. 
     
     
         24 . A method for treating pre-ensiled plant material to enhance the digestibility of the resulting silage, which comprises: adding to said pre-ensiled plant material a digestibility enhancing amount of the composition of  claim 3 . 
     
     
         25 . The method of  claim 24 , wherein the pre-ensiled plant material is selected from the group consisting of grasses, maize, alfalfa, wheat, legumes, sorghum, sunflower, barley and mixtures thereof.

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