Fluorescence observation system and set of filters
Abstract
A set of filters for fluorescence observation comprises an illumination light filter and an observation light filter, wherein the following is holds: ∫ S T L ( r -> ) · T O ( r -> ) · r -> · r ∫ S T L ( r -> ) · T O ( r -> ) · r = R -> and ( 3 ) W -> - R -> ≤ 0.2 ; ( 4 ) wherein: λ designates the wavelength, T L (λ) is the transmission characteristic of the illumination light filter, T O (λ) is the transmission characteristic of the observation light filter, and A 1 , A 2 are numbers between 0 and 1, {right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, S is a line called the spectral locus in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and {right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.
Claims
exact text as granted — not AI-modified1 . A set of filters comprising an illumination light filter and an observation light filter;
wherein a transmission characteristic of the illumination light filter is a sum of a first partial characteristic and a second partial characteristic; wherein a transmission characteristic of the observation light filter is a sum of the third partial characteristic and a fourth partial characteristic; wherein the first partial characteristic has, at wavelengths below a threshold wavelength, at least one wavelength range in which the transmission has values greater than a first value; wherein the second partial characteristic has, at wavelengths above the threshold wavelength, at least one second wavelength range in which the transmission has values less than a second value and greater than a third value, wherein the transmission of the illumination light filter has, between the first wavelength range and the second wavelength range, values less than a fourth value; wherein the third partial characteristic has, at wavelengths above the threshold wavelength, at least one third wavelength range in which the transmission has values greater than the first value; wherein the fourth partial characteristic has, at wavelengths below the threshold wavelength, at least one fourth wavelength range in which the transmission has values less than the second value and greater than the third value; and wherein the fourth value is less than the third value, the third value is less than the second value and the second value is less than the first value.
2 . The set of filters according to claim 1 , wherein:
0
≤
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
3
(
λ
)
λ
<
A
1
and
(
1
)
2
A
1
<
1
300
nm
∫
400
nm
700
nm
T
L
(
λ
)
·
T
O
(
λ
)
·
λ
<
A
2
;
(
2
)
wherein:
λ designates the wavelength,
T 1 (λ) is the first partial characteristic,
T 2 (λ) is the second partial characteristic,
T 3 (λ) is the third partial characteristic,
T L (λ)=T 1 (λ)+T 2 (λ) is the transmission characteristic of the illumination light filter,
T O (λ) is the transmission characteristic of the observation light filter, and
A 1 , A 2 are numbers between 0 and 1.
3 . The set of filters according to claim 1 , wherein:
0
≤
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
3
(
λ
)
λ
<
A
1
;
2
A
1
<
1
300
nm
∫
400
nm
700
nm
T
L
(
λ
)
·
T
O
(
λ
)
·
λ
<
A
2
;
A
1
<
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
4
(
λ
)
·
λ
<
0.5
A
2
;
and
A
1
<
1
300
nm
∫
400
nm
700
nm
T
2
(
λ
)
·
T
3
(
λ
)
·
λ
<
0.5
A
2
;
wherein
λ designates the wavelength,
T 1 (λ) is the first partial characteristic,
T 2 (λ) is the second partial characteristic,
T 3 (λ) is the third partial characteristic,
T 4 (λ) is the fourth partial characteristic,
T L (λ)=T 1 (λ)+T 2 (λ) is the transmission characteristic of the illumination light filter,
T O (λ)=T 3 (λ)+T 4 (λ) is the transmission characteristic of the observation light filter, and
A 1 , A 2 are numbers between 0 and 1.
4 . A fluorescence observation system comprising:
a light source for illuminating an object; observation optics for imaging the object; an illumination light filter disposed in an illumination beam path between the light source and the object; and an observation light filter disposed in a beam path of the observation optics; wherein a transmission characteristic of the illumination light filter is a sum of a first partial characteristic and a second partial characteristic; wherein a transmission characteristic of the observation light filter is a sum of a third partial characteristic and a fourth partial characteristic; wherein the first partial characteristic has, at wavelengths below a threshold wavelength, at least one wavelength range in which the transmission has values greater than a first value; wherein the second partial characteristic has, at wavelengths above the threshold wavelength, at least one second wavelength range in which the transmission has values less than a second value and greater than a third value, wherein the transmission of the illumination light filter has, between the first wavelength range and the second wavelength range, values less than a fourth value; wherein the third partial characteristic has, at wavelengths above the threshold wavelength, at least one third wavelength range in which the transmission has values greater than the first value; wherein the fourth partial characteristic has, at wavelengths below the threshold wavelength, at least one fourth wavelength range in which the transmission has values less than the second value and greater than the third value, wherein the transmission of the observation light filter has, between the fourth wavelength range and the third wavelength range, values which are smaller than the fourth value; and wherein the fourth value is less than the third value, the third value is less than the second value and the second value is less than the first value.
5 . The fluorescence observation system according to claim 4 , wherein:
0
≤
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
3
(
λ
)
λ
<
A
1
;
2
A
1
<
1
300
nm
∫
400
nm
700
nm
T
L
(
λ
)
·
T
O
(
λ
)
·
λ
<
A
2
;
A
1
<
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
4
(
λ
)
·
λ
<
0.5
A
2
;
and
A
1
<
1
300
nm
∫
400
nm
700
nm
T
2
(
λ
)
·
T
3
(
λ
)
·
λ
<
0.5
A
2
;
wherein
λ designates the wavelength,
T 1 (λ) is the first partial characteristic,
T 2 (λ) is the second partial characteristic,
T 3 (λ) is the third partial characteristic,
T 4 (λ) is the fourth partial characteristic,
T L (λ)=T 1 (λ)+T 2 (λ) is the transmission characteristic of the illumination light filter,
T O (λ)=T 3 (λ)+T 4 (λ) is the transmission characteristic of the observation light filter, and
A 1 , A 2 are numbers between 0 and 1.
6 . A method of performing a fluorescence observation, wherein the method comprises:
filtering of an illumination light beam directed to an object using an illumination light filter, and filtering of light emanating from the object using an observation light filter; wherein a transmission characteristic of the illumination light filter is a sum of a first partial characteristic and a second partial characteristic; wherein a transmission characteristic of the observation light filter is a sum of a third partial characteristic and a fourth partial characteristic; wherein the first partial characteristic has, at wavelengths below a threshold wavelength, at least one wavelength range in which the transmission has values greater than a first value; wherein the second partial characteristic has, at wavelengths above the threshold wavelength, at least one second wavelength range in which the transmission has values less than a second value and greater than a third value, wherein the transmission of the illumination light filter has, between the first wavelength range and the second wavelength range, values less than a fourth value; wherein the third partial characteristic has, at wavelengths above the threshold wavelength, at least one third wavelength range in which the transmission has values greater than the first value; wherein the fourth partial characteristic has, at wavelengths below the threshold wavelength, at least one fourth wavelength range in which the transmission has values less than the second value and greater than the third value, wherein the transmission of the observation light filter has, between the fourth wavelength range and the third wavelength range, values which are smaller than the fourth value; and wherein the fourth value is less than the third value, the third value is less than the second value and the second value is less than the first value.
7 . The method according to claim 6 , wherein
0
≤
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
3
(
λ
)
λ
<
A
1
;
2
A
1
<
1
300
nm
∫
400
nm
700
nm
T
L
(
λ
)
·
T
O
(
λ
)
·
λ
<
A
2
;
A
1
<
1
300
nm
∫
400
nm
700
nm
T
1
(
λ
)
·
T
4
(
λ
)
·
λ
<
0.5
A
2
;
and
A
1
<
1
300
nm
∫
400
nm
700
nm
T
2
(
λ
)
·
T
3
(
λ
)
·
λ
<
0.5
A
2
;
wherein
λ designates the wavelength,
T 1 (λ) is the first partial characteristic,
T 2 (λ) is the second partial characteristic,
T 3 (λ) is the third partial characteristic,
T 4 (λ) is the fourth partial characteristic,
T L (λ)=T 1 (λ)+T 2 (λ) is the transmission characteristic of the illumination light filter,
T O (λ)=T 3 (λ)+T 4 (λ) is the transmission characteristic of the observation light filter, and
A 1 , A 2 are numbers between 0 and 1.Join the waitlist — get patent alerts
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