Variable domains of camelid heavy-chain antibodies directed against glial fibrillary acidic proteins

Abstract

The present invention relates to the use of variable domains of camelid heavy-chain antibodies (VHH domains) directed against an intracellular target and having an isoelectric point of at least 8.5, for targeting said intracellular target or for the preparation of a peptide vector. Particularly, it concerns VHH domains directed against a glial fibrillary acidic protein and uses thereof for preparing therapeutic or diagnostic agents.

1 .- 30 . (canceled) 
     
     
         31 . A method, comprising delivering a substance of interest into a mammal cell, wherein said mammalian cell comprises an intracellular target, using a peptide vector for said delivering step, and using a variable domain of a camelid heavy-chain antibody (VHH domain) directed against said intracellular target and having an isoelectric point of at least 8.5. 
     
     
         32 . The method of  claim 31 , wherein said VHH domain is hyperstable. 
     
     
         33 . The method of  claim 31 , wherein said VHH domain is directed against a glial fibrillary acidic protein (GFAP). 
     
     
         34 . The method of  claim 31 , wherein said mammal cell is an astrocyte.