Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . An isolated polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, selected from the group consisting of:
 (a) a polypeptide having at least 76% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 12;   (b) a polypeptide encoded by a polynucleotide that hybridizes under at least midium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 5 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 7 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 9 or the cDNA sequence thereof, or the mature polypeptide coding sequence of SEQ ID NO: 11 or the cDNA sequence thereof, or (ii) the full-length complement of (i);   (c) a polypeptide encoded by a polynucleotide having at least 76% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 5 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 7 or the cDNA sequence thereof, the mature polypeptide coding sequence of SEQ ID NO: 9 or the cDNA sequence thereof, or the mature polypeptide coding sequence of SEQ ID NO: 11 or the cDNA sequence thereof;   (d) a variant of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 12 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and   (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity.   
     
     
         2 . An isolated polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         3 . A recombinant host cell comprising the polynucleotide of  claim 2  operably linked to one or more control sequences that direct the production of the polypeptide. 
     
     
         4 . A method of producing the polypeptide of  claim 1 , comprising:
 (a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         5 . A method of producing a polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, comprising:
 (a) cultivating the host cell of  claim 3  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         6 . A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         7 . A method of producing a polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, comprising:
 (a) cultivating the transgenic plant or plant cell of  claim 6  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         8 . A method of producing a mutant of a parent cell, comprising inactivating a polynucleotide encoding the polypeptide of  claim 1 , which results in the mutant producing less of the polypeptide than the parent cell. 
     
     
         9 . An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 20 of SEQ ID NO: 2, amino acids 1 to 19 of SEQ ID NO: 4, amino acids 1 to 15 of SEQ ID NO: 6, amino acids 1 to 16 of SEQ ID NO: 8, amino acids 1 to 16 of SEQ ID NO: 10, or amino acids 1 to 23 of SEQ ID NO: 12. 
     
     
         10 . A recombinant host cell comprising a gene encoding a protein operably linked to the polynucleotide of  claim 9 , wherein the gene is foreign to the polynucleotide encoding the signal peptide. 
     
     
         11 . A process of producing a protein, comprising:
 (a) cultivating a recombinant host cell comprising a gene encoding a protein operably linked to the polynucleotide of  claim 9 , wherein the gene is foreign to the polynucleotide encoding the signal peptide, under conditions conducive for production of the protein; and   (b) recovering the protein.   
     
     
         12 . A whole broth formulation or cell culture composition comprising the polypeptide of  claim 1 . 
     
     
         13 . A process for degrading or converting a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity of  claim 1 . 
     
     
         14 . The process of  claim 13 , further comprising recovering the degraded cellulosic material or xylan-containing material. 
     
     
         15 . A process for producing a fermentation product, comprising:
 (a) saccharifying a cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity of  claim 1 ;   (b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product; and   (c) recovering the fermentation product from the fermentation.   
     
     
         16 . A process of fermenting a cellulosic material or xylan-containing material, comprising: fermenting the cellulosic material or xylan-containing material with one or more fermenting microorganisms, wherein the cellulosic material or xylan-containing material is saccharified with an enzyme composition in the presence of a polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity of  claim 1 . 
     
     
         17 . The process of  claim 16 , wherein the fermenting of the cellulosic material or xylan-containing material produces a fermentation product. 
     
     
         18 . The process of  claim 17 , further comprising recovering the fermentation product from the fermentation.