Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancer, diabetes, premenstrual syndrome and transdermal transport

Abstract

The present invention relates to a method of treatment and/or prevention of cardiovascular disease, rheumatoid arthritis, skin cancer, premenstrual syndrome, diabetes and transdermal transport enhancement. The method comprises the administration of a therapeutically effective amount of krill and/or marine oil to a patient. The present invention also relates to a composition for the treatment and/or prevention of these diseases.

1 - 57 . (canceled) 
     
     
         58 . A method for reducing cholesterol, comprising administrating a krill oil extract to a human subject, wherein:
 the krill oil is administered to the human subject in a quantity in a range of about 1 to about 4.8 grams per day, and   the krill oil is obtainable by a process comprising the steps of:
 a) placing krill in a ketone solvent to achieve extraction of the soluble lipid fraction from said krill; 
 b) separating the liquid and solid phases; 
 c) recovering a first lipid rich fraction from the liquid phase obtained at step (b) by evaporation of the solvent present in the liquid phase; 
 d) placing the solid phase in an organic solvent selected from the group consisting of alcohol and esters of acetic acid to achieve extraction of the remaining soluble lipid fraction from the solid phase; 
 e) separating the liquid and solid phases; and 
 f) recovering a second lipid rich fraction from the liquid phase obtained at step (e) by evaporation of the solvent present in the liquid phase. 
   
     
     
         59 . The method of  claim 58 , wherein the ketone solvent used in step (a) of the process is acetone. 
     
     
         60 . The method of  claim 58 , wherein the organic solvent used in step (d) of the process is selected from ethanol, isopropanol and t-butanol. 
     
     
         61 . The method of  claim 58 , wherein the organic solvent used in step (d) of the process is ethyl acetate. 
     
     
         62 . The method of  claim 58 , wherein the quantity is about 1 gram per day. 
     
     
         63 . The method of  claim 58 , wherein said krill oil comprises Eicosapentanoic acid, Docosahexanoic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, α-tocopherol, all-trans retinol, Astaxanthin and flavonoid. 
     
     
         64 . The method of  claim 58 , wherein said krill oil comprises Eicosapentanoic acid, Docosahexanoic acid, Linolenic acid, α-linolenic acid, Arachidonic acid, oleic acid, palmitic acid, palmitoleic acid, stearic acid, nervonic acid, Phosphatidylcholine, Phosphatidylinositol, Phosphatidylserine, Phosphatidylethanolamine, Sphingomyelin, cholesterol, triglycerides, monoglycerides, α-tocopherol, all-trans retinol, Astaxanthin, canthaxanthin, β-carotene, flavonoid, zinc, selenium, sodium, potassium and calcium. 
     
     
         65 . The method of  claim 58 , wherein said patient is a patient suffering from hyperlipidemia. 
     
     
         66 . The method of  claim 58 , wherein said composition is administered orally. 
     
     
         67 . The method of  claim 62 , wherein said composition is administered orally.