Steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

Abstract

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

What is claimed is: 
     
         1 . An isolated consortium of microbial species wherein said consortium comprises;
 a) at least one first species of the genus Thauera having a 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to SEQ ID NO: 15;   b) at least one second species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs:16, 19, 21, 23, 24-28, 30-41, 67 and 68; and   c) at least one third species having a 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 17, 18, 20, 22, 29, 54, 69 and 86 and combinations thereof.   
     
     
         2 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequences selected from the group consisting of SEQ ID NOs: 55, 63, 75, 76 and 81 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 42, 45, 50-52, 64-66 and 79 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 48, 49 and 82. 
     
     
         3 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 53, 58 and 87 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 56 and 77. 
     
     
         4 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 43. 
     
     
         5 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 60-62, 80 and 83 and having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 20, 44, 46, 57, 70-74, 84 and 85. 
     
     
         6 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs: 47 and 59. 
     
     
         7 . The isolated consortium of microbial species of  claim 1  further comprising species having 16S rDNA nucleic acid molecule having the nucleic acid sequence that has at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 78.