Thermus brockianus nucleic acid polymerases

Abstract

The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus . The invention also provides methods for using these nucleic acids and polypeptides.

1 . An isolated nucleic acid encoding a nucleic acid polymerase comprising any one of amino acid sequences SEQ ID NO:9-16. 
     
     
         2 . The isolated nucleic acid of  claim 1 , wherein said nucleic acid polymerase comprises a mutation that decreases 5-3′ exonuclease activity. 
     
     
         3 . The isolated nucleic acid of  claim 2 , wherein said decreased 5-3′ exonuclease activity is relative to a nucleic acid polymerase that does not comprise said mutation. 
     
     
         4 . The isolated nucleic acid of  claim 1 , wherein said nucleic acid polymerase comprises a mutation that reduces discrimination against dideoxynucleotide triphosphates. 
     
     
         5 . The isolated nucleic acid of  claim 4 , wherein said reduced discrimination against dideoxynucleotide triphosphates is relative to a nucleic acid polymerase that does not comprise said mutation. 
     
     
         6 . An isolated nucleic acid comprising the nucleotide sequence of any one of SEQ ID NO:1-8 or a nucleotide sequence complementary to any one of SEQ ID NO:1-8. 
     
     
         7 . (canceled) 
     
     
         8 . An isolated nucleic acid encoding a nucleic acid polymerase from  Thermus brockianus  comprising amino acid sequence with at least 96% identity to any one of SEQ ID NO:9-16. 
     
     
         9 . A vector comprising the isolated nucleic acid of  claim 8 . 
     
     
         10 .- 16 . (canceled) 
     
     
         17 . An expression vector comprising a promoter operably linked to the isolated nucleic acid of  claim 8 . 
     
     
         18 .- 24 . (canceled) 
     
     
         25 . A host cell comprising the isolated nucleic acid of  claim 8 . 
     
     
         26 .- 54 . (canceled)