Method for immobilizing protein a on a self-assembled monolayer

Abstract

The object of the present invention is to provide a method for increasing an amount of Protein A to be immobilized on the self-assembled monolayer. Immobilizing Protein A to the self-assembled monolayer through the structure represented following formula (II) obviates the object.

1 . A sensor comprising a self-assembled monolayer, a one molecule of the amino acid, and a Protein A, wherein,
 the one molecule of the amino acid is interposed between the selfassembled monolayer and the Protein A,   the Protein A is bound to the self-assembled monolayer through two peptide bonds represented by the following chemical formula (II),   
       
         
           
           
               
               
           
         
       
       wherein R represents a side chain of one molecule of the amino acid; 
       the one molecule of the amino acid is selected from the nineteen kinds of amino acids consisting of cysteine, lysine, histidine, phenylalanine, tyrosine, glycine, asparagine, methionine, serine, tryptophan, leucine, glutamine, alanine, isoleucine, threonine, proline, glutamate, aspartate, and valine. 
     
     
         2 . A sensor according to  claim 1 , wherein the chemical formula (II) is represented by the following chemical formula (III); 
       
         
           
           
               
               
           
         
         wherein R represents a side chain of one molecule of the amino acid. 
       
     
     
         3 . A sensor according to  claim 1 , wherein the one molecule of the amino acid is selected from the group consisting of cysteine, lysine, histidine, phenylalanine, tyrosine, glycine, asparagine, methionine, serine, tryptophan, leucine, glutamine, alanine, isoleucine, threonine, proline, glutamate, and aspartate. 
     
     
         4 . A sensor according to  claim 1 , wherein the one molecule of the amino acid is selected from the group consisting of cysteine, lysine, histidine, phenylalanine, tyrosine, glycine, asparagine, methionine, serine, tryptophan, leucine, glutamine, alanine, isoleucine, and threonine. 
     
     
         5 . A sensor according to  claim 1 , wherein the one molecule of the amino acid is selected from the group consisting of cysteine, lysine, histidine, phenylalanine, tyrosine, glycine, asparagine, methionine, serine, and tryptophan. 
     
     
         6 . A sensor according to  claim 1 , wherein the one molecule of the amino acid is selected from the group consisting of cysteine, lysine, histidine, phenylalanine, and glycine.