Targeted antimicrobial moieties

Abstract

This invention provides novel targeted antimicrobial compositions. In various embodiments chimeric moieties are provided comprising an antimicrobial peptide attached to a peptide targeting moiety that binds a bacterial strain or species.

1 . A chimeric moiety, said moiety comprising:
 an effector attached to a peptide targeting moiety the amino acid sequence of said targeting moiety comprising the sequence GGSGSLSTFFRLFNRSFTQALGK (SEQ ID NO:60).   
     
     
         2 . The chimeric moiety of  claim 1 , wherein the amino acid sequence of said targeting moiety is GGSGSLSTFFRLFNRSFTQALGK (SEQ ID NO:60). 
     
     
         3 . The chimeric moiety of  claim 1 , wherein said effector comprises a moiety selected from the group consisting of a detectable label, an antimicrobial peptide, an antibiotic, and a photosensitizer. 
     
     
         4 . The chimeric moiety of  claim 1 , wherein said effector comprises an antimicrobial peptide comprising the amino acid sequence of a peptide found in Table 2, Table 8, Table 9, or Table 10 or any antimicrobial peptide disclosed herein. 
     
     
         5 . The chimeric moiety of  claim 1 , wherein said effector comprises an antibiotic found in Table 7. 
     
     
         6 - 14 . (canceled) 
     
     
         15 . The chimeric moiety of  claim 1 , wherein said targeting moiety is chemically conjugated to said effector. 
     
     
         16 . The chimeric moiety of  claim 15 , wherein said targeting moiety is chemically conjugated to said effector via a linker. 
     
     
         17 . The chimeric moiety of  claim 15 , wherein said targeting moiety is chemically conjugated to said effector via a linker comprising a polyethylene glycol (PEG). 
     
     
         18 . The chimeric moiety of  claim 15 , wherein said targeting moiety is chemically conjugated to said effector via a non-peptide linker found in Table 11. 
     
     
         19 . The chimeric moiety of  claim 1 , wherein said targeting moiety is linked directly to said effector. 
     
     
         20 . The chimeric moiety of  claim 1 , wherein said targeting moiety is linked to said effector via a peptide linkage. 
     
     
         21 . The chimeric moiety of  claim 20 , wherein the chimeric moiety is a fusion protein. 
     
     
         22 . The chimeric moiety of  claim 21 , wherein said peptide linkage comprises a peptide linker found in Table 11. 
     
     
         23 . The chimeric moiety of  claim 20 , wherein said chimeric moiety is functionalized with a polymer to increase serum halflife. 
     
     
         24 . The chimeric moiety of  claim 21 , wherein said polymer comprises polyethylene glycol and/or a cellulose or modified cellulose. 
     
     
         25 . A pharmaceutical composition comprising a chimeric moiety of  claim 1  in a pharmaceutically acceptable carrier. 
     
     
         26 . The composition of  claim 25 , wherein said composition is formulated as a unit dosage formulation. 
     
     
         27 . The composition of  claim 25 , wherein said composition is formulated for administration by a modality selected from the group consisting of intraperitoneal administration, topical administration, oral administration, inhalation administration, transdermal administration, subdermal depot administration, and rectal administration. 
     
     
         28 - 45 . (canceled) 
     
     
         46 . The chimeric moiety of  claim 1 , wherein said effector comprises a photosensitizing agent. 
     
     
         47 . (canceled) 
     
     
         48 . The chimeric moiety of  claim 46 , wherein said photosensitizing agent is an agent selected from the group consisting of a porphyrinic macrocycle, a porphyrin, a chlorine, a crown ether, an acridine, an azine, a phthalocyanine, a cyanine, a psoralen, and a perylenequinonoid. 
     
     
         49 . The chimeric moiety of  claim 46 , wherein said photosensitizing agent is an agent shown in any of  FIGS. 1-11 . 
     
     
         50 . The chimeric moiety of  claim 46 , wherein said photosensitizing agent is attached to said targeting peptide by a non-peptide linker. 
     
     
         51 . The chimeric moiety of  claim 46 , wherein said photosensitizing agent is attached to said targeting peptide by a linker comprising a polyethylene glycol (PEG). 
     
     
         52 . The chimeric moiety of  claim 46 , wherein said photosensitizing agent is attached to said targeting peptide by a non-peptide linker found in Table 11. 
     
     
         53 - 58 . (canceled)