Plant transcription factors

Abstract

This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.

What is claimed is: 
     
         1 . An isolated polynucleotide comprising:
 (a) a nucleotide sequence encoding a polypeptide having LEC1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% sequence identity based on the Clustal alignment method, or   (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.   
     
     
         2 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 92% identity based on the Clustal alignment method. 
     
     
         3 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 94% identity based on the Clustal alignment method. 
     
     
         4 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 96% identity based on the Clustal alignment method. 
     
     
         5 . The polynucleotide of  claim 1 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 98% identity based on the Clustal alignment method. 
     
     
         6 . The polynucleotide of  claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30. 
     
     
         7 . The polynucleotide of  claim 1  wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, or 29. 
     
     
         8 . A vector comprising the polynucleotide of  claim 1 . 
     
     
         9 . A recombinant DNA construct comprising the polynucleotide of  claim 1  operably linked to a regulatory sequence. 
     
     
         10 . A method for transforming a cell, comprising transforming a cell the polynucleotide of  claim 1 . 
     
     
         11 . A cell comprising the recombinant DNA construct of  claim 9 . 
     
     
         12 . A method for producing a plant comprising transforming a plant cell with the polynucleotide of  claim 1  and regenerating a plant from the transformed plant cell. 
     
     
         13 . A plant comprising the recombinant DNA construct of  claim 9 . 
     
     
         14 . A seed comprising the recombinant DNA construct of  claim 9 . 
     
     
         15 . An isolated polynucleotide comprising a first nucleotide sequence, wherein the first nucleotide sequence contains at least 30 nucleotides, and wherein the first nucleotide sequence is comprised by another polynucleotide, wherein the other polynucleotide includes:
 (a) a second nucleotide sequence, wherein the second nucleotide sequence encodes a polypeptide having Lec1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% sequence identity based on the Clustal alignment method, or   (b) the complement of the second nucleotide sequence, wherein the complement and the second nucleotide sequence contain the same number of nucleotides and are 100% complementary.   
     
     
         16 . An isolated polypeptide having Lec1-related transcription factor activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 90% identity based on the Clustal alignment method. 
     
     
         17 . The polypeptide of  claim 16 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 92% identity based on the Clustal alignment method. 
     
     
         18 . The polypeptide of  claim 16 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 94% identity based on the Clustal alignment method. 
     
     
         19 . The polypeptide of  claim 16 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 96% identity based on the Clustal alignment method. 
     
     
         20 . The polypeptide of  claim 16 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30 have at least 98% identity based on the Clustal alignment method. 
     
     
         21 . The polypeptide of  claim 16 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 or 30. 
     
     
         22 . A method for isolating a polypeptide encoded by the polynucleotide of  claim 1  comprising isolating the polynucleotide from a cell containing a recombinant DNA construct comprising the polynucleotide operably linked to a regulatory sequence.