Wise/sost nucleic acid sequences and amino acid sequences

Abstract

The present invention relates to nucleic acid sequences and amino acid sequences which influence bone deposition, the Wnt pathway, ocular development, tooth development, and may bind to LRP. The nucleic acid sequence and polypeptides include Wise and Sost as well as a family of molecules which express a cysteine knot polypeptide. Additionally, the present invention relates to various molecular tools derived from the nucleic acids and polypeptides including vectors, transfected host cells, monochronal antibodies, Fab fragments, and methods for impacting the pathways.

1 - 181 . (canceled) 
     
     
         182 . A method for affecting tooth development comprising:
 (a) isolating Sost nucleic acid sequence;   (b) attaching a stop code at the beginning of the Sost nucleic acid sequence to form a Sost cassette;   (c) forming a Sost plasmid by inserting the Sost cassette into the plasmid;   (d) transfecting a host cell with the Sost plasmid, whereby homologous recombination occurs with a wild type Sost gene; and   (e) activating the stop code to cause a loss of function mutation.   
     
     
         183 - 235 . (canceled)