Genetic loci associated with iron deficiency tolerance in soybean

Abstract

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to iron deficient growth conditions. The methods use molecular genetic markers to identify, select and/or construct disease-tolerant plants or identify and counterselect disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Phytophthora root rot infection that are generated by the methods of the invention are also a feature of the invention.

What is claimed is: 
     
         1 . A method of identifying a first soybean plant or germplasm that displays tolerance, improved tolerance or susceptibility to iron deficiency, the method comprising detecting in the first soybean plant or germplasm at least one allele of one or more marker locus that is associated with the tolerance, improved tolerance or susceptibility, wherein the one or more marker locus is selected from the group consisting of: (a) S60210-TB, SAC1006, SATT391, SAC1724, SATT307, P13073A-1, P10598A-1, SATT334, SATT510, SATT335, P5219A-1, P7659A-2, SAT — 117, SATT191, S60143-TB, SATT451, SATT367, SATT495, P10649C-3, SATT613, SATT257, SATT581 and SATT153; (b) a marker locus closely linked to a marker locus of (a); (c) a marker locus localizing within a chromosome interval flanked by and including S60210-TB and SATT391; (d) a marker locus localizing within a chromosome interval flanked by and including P10598A-1 and SATT334; (e) a marker locus localizing within a chromosome interval flanked by and including SATT510 and SATT335; (f) a marker locus localizing within a chromosome interval flanked by and including P5219A-1 and P7659A-2; (g) a marker locus localizing within a chromosome interval flanked by and including SAT — 117 and S60143-TB; (h) a marker locus localizing within a chromosome interval flanked by and including SATT451 and STT367; (i) a marker locus localizing within a chromosome interval flanked by and including SATT495 and P10649C-3; and a marker locus localizing within a chromosome interval flanked by and including SATT250 and SATT346. 
     
     
         2 . The method of  claim 1 , wherein the closely linked marker locus of (b) displays a genetic recombination frequency of less than about 10% with the marker locus of (a). 
     
     
         3 . The method of  claim 1 , wherein the one or more marker locus of (a) is selected from the group consisting of SAC1724, SATT307, P13073A-1, P10598A-1, SATT334, SATT495, P10649C-3, SATT613 and SATT257. 
     
     
         4 . The method of  claim 1 , wherein the one or more marker locus associated with tolerance, improved tolerance or susceptibility is selected from the marker loci of (a) and (b). 
     
     
         5 . The method of  claim 1 , wherein the one or more marker locus associated with tolerance, improved tolerance or susceptibility is a plurality of loci selected from the marker loci of (a) and (b). 
     
     
         6 . The method of  claim 1 , wherein the one or more marker locus associated with tolerance, improved tolerance or susceptibility is selected from marker loci localizing within the chromosome intervals of (c), (d), (e), (f), (g), (h), (i) and (j). 
     
     
         7 . The method of  claim 1 , wherein the one or more marker locus associated with tolerance, improved tolerance or susceptibility is a plurality of loci selected from marker loci localizing within the chromosome intervals of (c), (d), (e), (f), (g), (h), (i) and (j). 
     
     
         8 . The method of  claim 1 , wherein the germplasm is a soybean line or soybean variety. 
     
     
         9 . The method of  claim 1 , wherein the tolerance, improved tolerance or susceptibility is assayed in a population of soybean in a stand that is known to produce chlorotic soybean plants. 
     
     
         10 . The method of  claim 1 , wherein the detecting comprises detecting at least one allelic form of a polymorphic simple sequence repeat (SSR) or a single nucleotide polymorphism (SNP). 
     
     
         11 . The method of  claim 1 , wherein the detecting comprises amplifying the marker locus or a portion of the marker locus and detecting the resulting amplified marker amplicon. 
     
     
         12 . A plant comprising in its genome one or more locus related to tolerance or susceptibility to iron deficiency, wherein the one or more locus is within a chromosome interval flanked by and including SATT334 and SATT510 or a chromosome interval flanked by and including SATT277 and SATT433. 
     
     
         13 . A plant comprising in its genome one or more locus related to tolerance or susceptibility to iron deficiency, wherein the one or more locus is closely linked to a marker selected from the group consisting of SATT334, SCT — 033, SAT — 120, SATT510, SAC1724, SATT319, SAT — 142-DB, SATT708-TB, SATT460, P13073A-1, and SATT307. 
     
     
         14 . The plant of  claim 12 , wherein the plant is a soybean line or soybean variety. 
     
     
         15 . The plant of  claim 13 , wherein the plant is a soybean line or soybean variety. 
     
     
         16 . The plant of  claim 12 , wherein the tolerance or susceptibility is assayed in a population of soybean in a stand that is known to produce chlorotic soybean plants. 
     
     
         17 . The plant of  claim 13 , wherein the tolerance or susceptibility is assayed in a population of soybean in a stand that is known to produce chlorotic soybean plants. 
     
     
         18 . The plant of  claim 12 , wherein the plant comprises an elite soybean strain or an exotic soybean strain. 
     
     
         19 . The plant of  claim 13 , wherein the plant comprises an elite soybean strain or an exotic soybean strain. 
     
     
         20 . A field comprising a plurality of plants of  claim 12 . 
     
     
         21 . A field comprising a plurality of plants of  claim 13 . 
     
     
         22 . A seed of the plant of  claim 12 . 
     
     
         23 . A seed of the plant of  claim 13 . 
     
     
         24 . A method of producing a plant with tolerance or susceptibility to iron deficiency, the method comprising planting the seed of  claim 22  in soil and growing a plant therefrom. 
     
     
         25 . A method of producing a plant with tolerance or susceptibility to iron deficiency, the method comprising planting the seed of  claim 23  in soil and growing a plant therefrom.