Insulin analogues of prolonged activity

Abstract

New biosynthetic analogues of recombined human insulin of prolonged therapeutical activity, which can find place in prophylactic and treatment of diabetes.

1 . Insulin derivative or its pharmaceutically acceptable salt containing two polypeptides forming chain A and chain B, characterised by being a new polypeptide of formula 1: 
       
         
           
           
               
               
           
         
         where R denotes group of formula Asn-R 2 , where R 2  denotes group NH 2 ; 
         and R 1  denotes B31 Lys-B32Arg or B31Arg, wherein amino acid sequence of chain A is SEQ ID NO: 4, while amino acid sequence of chain B has been chosen form SEQ ID NO: 7 and 9, and wherein the new polypeptide of formula 1 is an analogue of recombined human insulin of isoelectric point 5-8.5. 
       
     
     
         2 . Pharmaceutical composition, characterised in that it contains effectively acting amount of insulin derivative or its pharmaceutically acceptable salt according to  claim 1 . 
     
     
         3 . Pharmaceutical composition according to  claim 2 , characterised in that it contains 10 up to 50 μg/ml of zinc.