High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis

Abstract

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

1 . A composition for separating analytes by capillary electrophoresis comprising:
 a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Daltons (Da) and 3,000,000 Da; and   a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of C 1  to C 3  alkyl, halo-substituted C 1  to C 3  alkyl, methoxy-substituted C 1  to C 3  alkyl, and hydroxyl-substituted C 1  to C 3  alkyl; wherein the sieving component and the surface interaction component are the same or different;   wherein the composition does not include a crosslinked polymer gel.   
     
     
         2 . The composition according to  claim 1 , wherein the composition has a viscosity of less than 10,000 centipoise at 25° C. 
     
     
         3 . The composition according to  claim 1 , wherein the composition has a viscosity of less than 5,000 centipoise at 25° C. 
     
     
         4 . The composition according to  claim 1 , wherein the composition has a viscosity of less than 1000 centipoise at 25° C. 
     
     
         5 . The composition according to  claim 1 , wherein the composition has a viscosity of less than 600 centipoise at 25° C. 
     
     
         6 . The composition of  claim 1 , wherein the one or more non-crosslinked polymers comprises poly(N,N-dimethylacrylamide). 
     
     
         7 . The composition of  claim 1 , further comprising at least one denaturant. 
     
     
         8 . The composition of  claim 7 , wherein the at least one denaturant is selected from the group consisting of at least one of formamide, urea, and 2-pyrollidinone. 
     
     
         9 . The composition of  claim 8 , wherein the at least one denaturant comprises urea. 
     
     
         10 . A composition for separating analytes by capillary electrophoresis comprising:
 a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and having a viscosity of less than 10,000 centipoise at 25° C.;   a surface interaction component comprising poly(N,N-dimethylacryiamide); and   a denaturant comprising urea;   wherein the composition does not include a crosslinked polymer gel.   
     
     
         11 . The composition of  claim 10 , wherein the viscosity is less than 5,000 centipoise at 25° C. 
     
     
         12 . The composition of  claim 10 , wherein the viscosity is less than 1,000 centipoise at 25° C. 
     
     
         13 . The composition of  claim 10 , wherein the viscosity is less than 600 centipoise at 25° C. 
     
     
         14 . A capillary electrophoresis element comprising:
 an uncoated capillary;   a composition for separating analytes located within the uncoated capillary, the composition comprising: a sieving component comprising a uncrosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and having a viscosity of less than 10,000 centipoise at 25° C.; and a surface interaction component;   wherein the surface interaction component comprises a solution of one or more non-crosslinked polymers; and   wherein the capillary electrophoresis element does not include a crosslinked polymeric gel.   
     
     
         15 . (canceled) 
     
     
         16 . The capillary electrophoresis element of  claim 14 , wherein the composition has a viscosity of less than 1000 centipoise at 250 C. 
     
     
         17 . (canceled) 
     
     
         18 . The capillary electrophoresis element of  claim 14 , wherein the one or more surface interaction component non-crosslinked polymers are selected from the group consisting of N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of C 1  to C 3  alkyl, halo-substituted C 1  to C 3  alkyl, methoxy-substituted C 1  to C 3  alkyl, and hydroxyl-substituted C 1  to C 3  alkyl. 
     
     
         19 . The capillary electrophoresis element of  claim 18 , wherein the surface interaction component non-crosslinked polymer is poly(N,N-dimethylacrylamide). 
     
     
         20 .- 50 . (canceled) 
     
     
         51 . A kit for separating analytes by capillary electrophoresis comprising a composition comprising a sieving component comprising an non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and a viscosity of less than 1000 centipoise at 25° C., wherein the composition does not include a crosslinked polymer gel. 
     
     
         52 . A kit according to  claim 51 , further comprising a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of C 1  to C 3  alkyl, halo-substituted C 1  to C 3  alkyl, methoxy-substituted C 1  to C 3  alkyl, and hydroxyl-substituted C 1  to C 3  alkyl. 
     
     
         53 . A kit according to  claim 52 , wherein the surface interaction component is poly(N,N-dimethylacrylamide). 
     
     
         54 .- 56 . (canceled)