Composition for the attachment of implants to collagen or other components of biological tissue

Abstract

The present invention relates to a novel composition comprising an implant, scaffold or construct bound to a biological or chemical moiety. The bound moiety has the ability to bind to a component of the extracellular matrix of biological tissue, allowing the implant to be bound to the biological tissue in a short period of time after implantation. The invention also relates to the use and manufacture of this novel composition, as well as a novel use for the protein CNA.

1 - 24 . (canceled) 
     
     
         25 . A composition comprising an implant and a biological moiety derived from bacteria or a chemical moiety, wherein the biological or chemical moiety is bound to the implant and has the ability to bind to a component of the extracellular matrix of a biological tissue, whereby the implant and the biological tissue become attached upon introduction of the composition to biological tissue. 
     
     
         26 . The composition of  claim 25 , wherein the biological moiety is a protein, protein sub-domain or mutated protein. 
     
     
         27 . The composition of  claim 26  wherein the protein, protein sub-domain or mutated protein has an increased ability to bind to the component of the extracellular matrix of the biological tissue. 
     
     
         28 . The composition of  claim 26 , wherein the protein is a mammalian collagen binding protein. 
     
     
         29 . The composition of  claim 26 , wherein the protein is derived from  Staphylococcus aureus, Enterococcus faecalis , or  Streptococcus mutans.    
     
     
         30 . The composition of  claim 25 , wherein the biological tissue is musculoskeletal tissue. 
     
     
         31 . The composition of  claim 25 , wherein the implant is comprised of a non-biodegradable, partly degradable or fully degradable polymer. 
     
     
         32 . The composition of  claim 25 , wherein the implant is comprised of poly(vinyl) alcohol. 
     
     
         33 . The composition of  claim 25 , wherein the implant is porous. 
     
     
         34 . The composition of  claim 25 , wherein the implant allows for the migration of cells into the implant. 
     
     
         35 . The composition of  claim 25 , wherein the implant is fibrin or fibrinogen. 
     
     
         36 . The composition of  claim 25 , further comprising a growth factor or chemoattractant. 
     
     
         37 . A composition comprising an implant and a biological moiety derived from bacteria or a chemical moiety, wherein the biological or chemical moiety is bound to the implant and has the ability to bind to collagen in biological tissue, whereby the implant and the biological tissue become attached upon introduction of the composition to biological tissue. 
     
     
         38 . A method of using the composition of  claim 37  for the treatment, repair or replacement of musculoskeletal tissue, by implantation of the composition into a subject in need thereof. 
     
     
         39 . The method of  claim 37 , wherein the subject is a mammal. 
     
     
         40 . The method of  claim 39 , wherein the mammal is a human. 
     
     
         41 . A method of using the composition of  claim 37  for the treatment, repair or replacement of musculoskeletal tissue, by implantation of the composition into a subject in need thereof. 
     
     
         42 . A method for the manufacture of the composition comprising an implant and a biological or chemical moiety of  claim 25 , comprising the steps of:
 a. lyophilizing the implant for a period of time sufficient to remove all of the water from the implant without collapsing the porous structure;   b. exposing the implant to a vapor of zirconium tetra(tert-butoxide);   c. heating the implant;   d. agitating the implant in the presence of zero grade nitrogen;   e. re-exposing the implant to a vapor of zirconium tetra(tert-butoxide);   f. heating and agitating the implant in the presence of zero grade nitrogen;   g. exposing the implant to 11-phosphonoundecanol;   h. submerging the implant in p-nitrophenyl chloroformate dissolved in tetrahydrofuran;   i. adding diisopropylethylamine to the solution containing the implant; and   j. removing the implant;   
       wherein the resulting composition comprises the implant and a chemical moiety bound to the implant. 
     
     
         43 . The method of  claim 42 , further comprising the step of soaking the implant in a solution of a biological or chemical moiety for a period of time sufficient for the chemical or biological moiety to bind to the implant.