US2014048416A1PendingUtilityA1

Systems and methods for single-molecule detection using nanopores

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Assignee: UNIV COLUMBIAPriority: Feb 23, 2011Filed: Jul 15, 2013Published: Feb 20, 2014
Est. expiryFeb 23, 2031(~4.6 yrs left)· nominal 20-yr term from priority
Inventors:Jacob RosensteinKenneth L. Shepard
G01N 27/44713G01N 27/44791G01N 33/48721Y10S977/852G01N 33/68C12Q 1/6869B82Y 5/00G01N 27/44704G01N 27/447B82Y 15/00
59
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Claims

Abstract

A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

Claims

exact text as granted — not AI-modified
1 . A method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof, comprising:
 passing a target molecule through the nanopore; and   measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.   
     
     
         2 . The method of  claim 1 , wherein the preamplifier comprises a low-noise multi-channel CMOS preamplifier. 
     
     
         3 . The method of  claim 1 , wherein the electrode is selected from the group consisting of Ag/AgCl, platinum, gold, and un-chlorinated silver. 
     
     
         4 . The method of  claim 1 , wherein the nanopore is positioned on a silicon chip adjacent to the preamplifier. 
     
     
         5 . The method of  claim 1 , wherein the nanopore comprises a nanopore directly integrated onto the preamplifier. 
     
     
         6 . The method of  claim 5 , wherein the first and second reservoirs are located on opposite sides of the preamplifier. 
     
     
         7 . The method of  claim 1 , wherein the nanopore comprises a solid state nanopore. 
     
     
         8 . The method of  claim 1 , wherein the nanopore comprises a biological nanopore. 
     
     
         9 . The method of  claim 8 , wherein the membrane is a lipid bilayer. 
     
     
         10 . The method of  claim 1 , wherein the electrode is in direct contact with one of the first and second reservoirs. 
     
     
         11 . The method of  claim 1 , wherein the integrated circuit comprises a plurality of nanopores. 
     
     
         12 . An integrated circuit for single-molecule detection, comprising:
 at least one membrane having a nanopore located between first and second reservoirs;   a low-noise preamplifier for measuring the change in pore current upon passing a target entity through the nanopore, wherein the preamplifier has at least one electrode formed on the surface thereof.   
     
     
         13 . The integrated circuit of  claim 12 , wherein the preamplifier comprises a low-noise multi-channel CMOS preamplifier. 
     
     
         14 . The integrated circuit of  claim 12 , wherein the electrode is selected from the group consisting of Ag/AgCl, platinum, gold, and un-chlorinated silver. 
     
     
         15 . The integrated circuit of  claim 12 , wherein the nanopore is located through a silicon chip adjacent to the preamplifier. 
     
     
         16 . The integrated circuit of  claim 12 , wherein the nanopore comprises a nanopore directly integrated onto the preamplifier. 
     
     
         17 . The integrated circuit of  claim 16 , wherein the first and second reservoirs are located on opposite sides of the preamplifier. 
     
     
         18 . The integrated circuit of  claim 12 , wherein the nanopore comprises a solid state nanopore. 
     
     
         19 . The integrated circuit of  claim 12 , wherein the nanopore comprises a biological nanopore. 
     
     
         20 . The integrated circuit of  claim 19 , wherein the membrane is a lipid bilayer. 
     
     
         21 . The integrated circuit of  claim 12 , wherein the electrode is in direct contact with one of the first and second reservoirs. 
     
     
         22 . The integrated circuit of  claim 12 , wherein the integrated circuit comprises a plurality of nanopores.

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