Microfluidic Devices and Methods

Abstract

Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

What is claimed is: 
     
         1 . A method for reducing mixing times associated with using a microfluidic device, the method comprising:
 providing a microfluidic device having a reaction cell, the reaction cell including a sample chamber having a first volume and a reagent chamber having a second volume, the sample chamber and the reagent chamber being in fluid communication through an interface channel having an interface valve for controlling fluid communication between the sample chamber and the reagent chamber;   introducing a sample fluid into the sample chamber so as to pressurize the sample fluid in the sample chamber to a first pressure;   introducing a reagent fluid into the reagent chamber so as to pressurize the reagent fluid in the reagent chamber to a second pressure; and   mixing sample fluid from the sample chamber with reagent fluid from the reagent chamber by opening the interface valve, wherein the first pressure and the second pressure are sufficiently different to cause the mixing to occur at least in part by fluid injection.