Spectroscopic finger-printing of raw materials

Abstract

The invention provides a method for the selection of cultivation component batches to be used in the cultivation of a mammalian cell expressing a protein of interest wherein at least two different components are employed in the cultivation.

1 . A method for the selection of cultivation component batches to be used in the cultivation of a mammalian cell expressing a protein of interest wherein at least two different components are employed in the cultivation comprising the following steps:
 a) providing spectra of different batches of a first component obtained with a first spectroscopic method selected from NIR spectroscopy and MRI spectroscopy and spectra of a second component obtained with 2D-fluorescence spectroscopy and the cultivation supernatant yield of the protein of interest obtained in a cultivation using combinations of these different batches of the first and the second component,   b) identifying a relation of fused spectra of the two different spectroscopic techniques after computing spectra PCA scores with the yield of the cultivation,   c) providing a spectrum of a further batch of the first component obtained with the first spectroscopic method and a spectrum of a further batch of the second component obtained with the second spectroscopic method,   d) selecting the combination of the provided first component and the provided second component if the predicted cultivation supernatant yield based on the relation of fused spectra after computing spectra PCA scores identified in b) is within +/−10% of the mean yield provided in a).   
     
     
         2 . canceled 
     
     
         3 . The method according to  claim 1 , characterized in that the identifying is by principal component analysis. 
     
     
         4 . The method according to  claim 3 , characterized in that the principal component analysis is an unfolded principal component analysis. 
     
     
         5 . The method according to  claim 4 , characterized in that the unfolding preserves the information of the first mode (sample). 
     
     
         6 . The method according to  claim 1 ,  3 ,  4  and  5 , characterized in that the identifying is of a relation between spectra fused and compressed with PCA scores with cultivation yield at harvest by partial least square analysis. 
     
     
         7 . The method according to  claim 1 ,  3 ,  4  and  5 , characterized in that the protein of interest is an antibody or an antibody fragment or an antibody conjugate.