Methods For Producing Secreted Polypeptides

Abstract

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.

What is claimed is: 
     
         1 . A nucleic acid construct comprising a first polynucleotide comprising a nucleotide sequence encoding a signal peptide operably linked to a second polynucleotide comprising a nucleotide sequence encoding a polypeptide, wherein the first polynucleotide encoding the signal peptide is foreign to the second polynucleotide encoding the polypeptide, and the 3′ end of the first polynucleotide encoding the signal peptide is immediately upstream of the initiator codon of the second polynucleotide encoding the polypeptide; wherein the nucleotide sequence encoding the signal peptide is:
 (a) a nucleotide sequence encoding a signal peptide comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 37; or 
 (b) a nucleotide sequence encoding a signal peptide comprising a sequence having at least 90% sequence identity to SEQ ID NO: 36. 
 
     
     
         2 . The nucleic acid construct of  claim 1 , wherein the nucleotide sequence encoding a signal peptide comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 37. 
     
     
         3 . The nucleic acid construct of  claim 2 , wherein the nucleotide sequence encoding a signal peptide comprises an amino acid sequence having at least 97% sequence identity to SEQ ID NO: 37. 
     
     
         4 . The nucleic acid construct of  claim 1 , wherein the nucleotide sequence encoding a signal peptide comprises a sequence having at least 95% sequence identity to SEQ ID NO: 36. 
     
     
         5 . The nucleic acid construct of  claim 4 , wherein the nucleotide sequence encoding a signal peptide comprises a sequence having at least 97% sequence identity to SEQ ID NO: 36. 
     
     
         6 . A recombinant expression vector comprising the nucleic acid construct of  claim 1 . 
     
     
         7 . A recombinant host cell comprising the nucleic acid construct of  claim 1 .