Method for Producing Probiotically Derived Compounds

Abstract

A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth.

I claim: 
     
         1 . A process for naturally deriving a beneficial compound comprising:
 preparing a microbiological culture comprising at least one live probiotic organism and at least one nutritive agent or at least one nutraceutical agent;   incubating the microbiological culture to initiate probiotic activity;   halting the probiotic activity;   harvesting a waste byproduct of the probiotic activity; and   separating the beneficial compound from the waste byproduct.   
     
     
         2 . The process of  claim 1  wherein the at least one live probiotic organism is selected from the group consisting of  Lactobacillus  species,  Bifidobacterium  species,  Enterococcus  species,  Streptococcus thermophilus , and combinations thereof. 
     
     
         3 . The process of  claim 1  wherein the microbiological culture is incubated at a temperature of from about 35° C. to about 40° C. 
     
     
         4 . The process of  claim 1  wherein the microbiological culture is incubated for a period of from about 24 hours to about 240 hours. 
     
     
         5 . The process of  claim 1  wherein the probiotic activity is halted by adding organic ethanol. 
     
     
         6 . A process for naturally deriving at least one B vitamin coenzyme comprising:
 preparing a microbiological culture comprising at least one live probiotic organism selected from the group consisting of  Lactobacillus  species,  Bifidobacterium  species,  Enterococcus  species,  Streptococcus thermophilus , and combinations thereof, at least one nutritive agent, and at least one source of a B vitamin;   incubating the microbiological culture to initiate probiotic activity;   halting the probiotic activity;   harvesting a waste byproduct of the probiotic activity; and   separating the beneficial compound from the waste byproduct.   
     
     
         7 . The process of  claim 6  wherein the nutritive agent comprises at least one species of nutritional yeast. 
     
     
         8 . The process of  claim 6  wherein the nutritive agent comprises  Saccharomyces cerevisiae.    
     
     
         9 . The process of  claim 6  wherein the at least one naturally derived B vitamin coenzyme is selected from the group consisting of 5-methyltetrahydofolate, 5-deoxyadenosylcobalamin, pyridoxal-5-phosphate, coenzyme A, inositol hexanicotinamide, riboflavin-5-phosphate, thiamin cocarboxylase, inositol, choline, biotin and combinations thereof. 
     
     
         10 . The process of  claim 6  wherein the microbiological culture is incubated for a period of from about 96 hours to about 240 hours. 
     
     
         11 . A process for naturally deriving a beneficial compound comprising:
 preparing a microbiological culture comprising at least one live probiotic organism selected from the group consisting of  Lactobacillus  species,  Bifidobacterium  species,  Enterococcus  species,  Streptococcus thermophilus , and combinations thereof and at least one nutraceutical agent;   incubating the microbiological culture to initiate probiotic activity;   halting the probiotic activity;   harvesting a waste byproduct of the probiotic activity; and   separating the beneficial compound from the waste byproduct.   
     
     
         12 . The process of  claim 11  wherein the naturally derived beneficial compound comprises at least one polyphenol compound. 
     
     
         13 . The process of  claim 12  wherein the nutraceutical agent comprises a material selected from the group consisting of green tea in whole, chopped or powdered form, at least one polyphenol concentrate, and combinations thereof. 
     
     
         14 . The process of  claim 12  wherein the at least one polyphenol compound is selected from the group consisting of epigallocatechin-3-gallate, epigallocatechin, epicatechin-3-gallate, epicatechin, catechin-3-galate, catechin and combinations thereof. 
     
     
         15 . The process of  claim 12  wherein the microbiological culture is incubated for a period of from about 96 hours to about 144 hours. 
     
     
         16 . The process of  claim 11  wherein the naturally derived beneficial compound is UVI-quinol. 
     
     
         17 . The process of  claim 16  wherein the nutraceutical agent comprises UVI-quinone. 
     
     
         18 . The process of  claim 16  wherein the microbiological culture further comprises at least one nutritive agent. 
     
     
         19 . The process of  claim 18  wherein the at least one nutritive agent comprises tumeric rhizome. 
     
     
         20 . The process of  claim 16  wherein microbiological culture is incubated for a period of from about 168 hours to about 196 hours.